In Vitro Cellular Senescence Model Customization Services
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In Vitro Cellular Senescence Model Customization Services

Cellular senescence is a complex biological process characterized by permanent growth arrest and a distinct senescence-associated phenotype. It plays a crucial role in aging and aging-related diseases. Cellular senescence models are experimental systems in which cells are induced to undergo senescence through various methods. These models enable researchers to investigate the molecular mechanisms, functional changes, and phenotypic characteristics associated with senescence. Customization of this model involves a targeted approach to address key questions and explore innovative strategies in the field of aging research.

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CD BioSciences is committed to delivering top-notch, customized services for cellular senescence models according to different cell types and cellular senescence induction methods, empowering our clients to advance their aging research and therapeutic discovery endeavors with precision and scientific excellence. We also specialize in developing cellular senescence models based on our client's specific requirements. Our services possess the flexibility to manipulate variables such as cell types, senescence markers, induction protocols, and experimental endpoints.

Customization Services by Cell Type

At CD BioSciences, we understand that different cell types exhibit unique characteristics and responses to cellular senescence induction stimuli. Our team of experts offers guidance on the advantages and limitations of each cell type to help researchers choose the appropriate model for their specific research goals. We employ a variety of well-established methods to induce cellular senescence, such as replicative exhaustion, genotoxic stress, oxidative stress, and oncogene activation. By customizing the senescence models based on cell type, researchers can gain insights into tissue-specific senescence mechanisms and address the relevance of senescence in various physiological and pathological contexts. We offer customized services of senescence models for a wide range of cell types, including but not limited to the following.

Senescent-Induced Fibroblasts

Senescent-Induced Epithelial Cells

Senescent-Induced Endothelial Cells

Senescent-Induced Chondrocytes

Senescent-Induced Myoblasts

Senescent-Induced Mesenchymal Stem Cells

Paracrine Senescent Cells

Overview

Modeling Methods

Fig. 1 Schematic illustration of senescent fibroblasts. (Noren Hooten N and Evans MK, 2017)

The development of therapeutic approaches to slow or reverse the adverse physiological and pathological changes associated with aging has been considered an important goal of gerontological research. As cellular senescence has been characterized as the basis of aging in organisms, the culture and subculture of normal human diploid fibroblasts to mimic in vivo aging processes has been developed as an important method to study the molecular events involved in aging.

CD BioSciences, a pioneer in aging research, offers a range of customization services for senescent fibroblasts, providing our clients with valuable tools to unravel the mechanisms underlying aging and explore potential anti-aging strategies.

At CD BioSciences, we employ various methods to induce senescence in fibroblasts, faithfully recapitulating the aging process in vitro. By simulating the key hallmarks of aging, our customized services enable clients to study the complex interplay of cellular and molecular events associated with aging.

  • UVB irradiation of fibroblast. UVB radiation triggers DNA damage and oxidative stress, activating cellular senescence pathways. We utilize controlled UVB irradiation to induce senescence in fibroblasts, contributing to the study of the process of photoaging in the skin. Our approach provides researchers with a valuable tool to investigate the impact of UV radiation on fibroblast aging and study the molecular mechanisms involved.
  • Accelerated proliferation of fibroblast. We provide services for constructing senescent fibroblast models by inducing cell proliferation. By culturing fibroblasts with optimized media for rapid cell division, we can induce replicative senescence more efficiently. Our approach allows clients to study the effects of accelerated aging on fibroblast behavior and explore potential interventions to counteract premature aging.
  • In vitro H2O2 induction. Our services help clients construct the H2O2 -induced premature senescence model effectively with appropriate cell-line type, maintaining procedures, dose, time of exposure, and type of solvent. We treat fibroblast cultures with varying concentrations of H2O2 for specific durations to mimic oxidative stress conditions found in aging cells.

Overview

Modeling Methods

Epithelial cells play a critical role in maintaining the integrity and function of various tissues and organs in the body. As these cells age, their functionality and regenerative capacity decline, leading to impaired tissue homeostasis and increased susceptibility to disease. Modeling senescence in epithelial cells is of paramount importance for understanding the underlying mechanisms and developing targeted interventions to promote healthy aging and prevent age-related diseases.

CD BioSciences is a leading company specializing in providing high-quality customization services for aging epithelial cells. With years of experience in the industry, we have developed a deep understanding of the unique challenges and requirements associated with studying aging in epithelial cells.

  • Hydrogen peroxide (H2O2) induction. By carefully calibrating the concentration and exposure time of H2O2, we can induce controlled levels of oxidative stress to help clients construct aging epithelial cells.
  • Exposure to ultraviolet (UV) radiation. Our experts model aging epithelial cells by exposing them to UVA and UVB, which can generate large amounts of reactive oxygen species (ROS) directly.
  • Doxorubicin induction of aging epithelial cells. In addition to oxidative stress induction, we also utilize doxorubicin, a chemotherapeutic agent, to induce senescence in epithelial cells. Our experienced team of scientists at CD BioSciences has optimized the doxorubicin induction protocols to ensure reproducibility and minimize cell-to-cell variability. Our services allow for an accurate and reliable assessment of the aging phenotype in cultured epithelial cells.

Overview

Modeling Methods

Fig. 1 Characteristics of senescent endothelial cells. (Hwang HJ, et al., 2022)

The endothelial cell (EC) monolayer forms the inner cellular lining of all blood vessels forming a critical interface between blood and tissue. Vascular endothelium is involved in physiological functions, which include regulation of blood fluidity, hemostasis and clotting, vascular tone, immune responses, inflammation, angiogenesis, and metabolism. Modeling aging ECs mimics the aging process in a controlled laboratory setting, enabling researchers to study age-related changes without the confounding factors present in in vivo models.

CD BioSciences is a leading company specializing in providing customization services for aging ECs. We offer tailored solutions to meet the unique research needs of scientists and researchers studying age-related diseases and the cellular mechanisms underlying EC senescence.

  • Induction of oxidative stress in ECs. At CD BioSciences, we have developed robust approaches to induce oxidative stress in ECs for studying the aging process. We carefully calibrate the concentration and exposure time of H2O2 to induce controlled levels of oxidative stress in ECs, which allows for the investigation of the molecular and cellular changes associated with senescence.
  • Induction of replicative senescence in ECs. With each cell division, telomeres gradually shorten until a critical threshold is reached, triggering cell cycle arrest and cellular senescence. We offer services for the long-term culture of ECs for induction of replicative senescence. Our approach enables clients to study telomere shortening in aging ECs and their implications in age-related diseases.
  • Induction senescence via Inflammatory signals in ECs. Inflammatory cytokine can stimulate the senescence-associated secretory phenotype (SASP) in ECs. This secretory phenotype involves the secretion of various factors that perpetuate senescence and contribute to tissue dysfunction. We offer customized services for exposing ECs to pro-inflammatory signals, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), allowing clients to investigate the molecular mechanisms involved in inflammatory-induced senescence and its implications in age-related diseases.
  • Induction of mechanical stress in Ecs. We provide services for subjecting ECs to controlled mechanical stress, such as high fluid shear stress or cyclic stretch. Our approaches can activate signaling pathways related to oxidative stress, inflammation, and mechanotransduction, ultimately leading to endothelial cell senescence.

Overview

Modeling Methods

Chondrocytes play a vital role in maintaining the structure and function of cartilage, which is crucial for joint health and mobility. As chondrocytes age, they experience changes in their morphology, functionality, and gene expression patterns, which can significantly impact the health and integrity of the surrounding cartilage tissue. Culturing aging chondrocytes enables researchers to study the effects of aging on the mechanical behavior and integrity of cartilage, contributing to a better understanding of age-related joint degeneration.

CD BioSciences is a leading company specializing in the field of longevity research and offers services in the customization of aging chondrocytes.

We employ advanced techniques to induce aging chondrocyte models. By replicating the natural aging process, we can study the cellular changes associated with senescence and develop targeted therapies.

  • Irradiation induction of chondrocytes. Exposure to ionizing radiation triggers DNA damage, leading to the activation of cellular senescence pathways. We help clients develop aging chondrocytes with precise and controlled exposure to irradiation, minimizing potential damage and maximizing the senescence induction efficiency. Our expertise in this area enables us to provide reliable and reproducible aging chondrocyte models for aging research and drug development.
  • Pro-inflammatory cytokine induction of chondrocytes. We utilize pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α), to induce senescence in chondrocytes and stimulate cellular responses that mimic the inflammatory microenvironment observed in aging joints. Our services assist clients in investigating the molecular mechanisms underlying senescence-related changes in cartilage.
  • Isolation from damaged cartilage to induce senescence in chondrocytes. We also offer a unique approach to inducing senescence in chondrocytes by isolating cells from damaged cartilage. In damaged joints, chondrocytes experience increased stress and mechanical loading, leading to accelerated senescence. By isolating these aging chondrocytes, our services enable clients to study their distinct characteristics and explore potential interventions to alleviate the aging-related effects on cartilage.

Overview

Modeling Methods

Myogenesis gradually deteriorates as the skeletal muscle ages, contributing to muscle mass loss. Indeed, one of the most serious consequences of aging is the progressive loss of muscle mass (sarcopenia) and function, which affects the quality of life and, ultimately, the survival of elderly people.

CD BioSciences specializes in providing a range of services for aging myoblasts. Our expertise lies in inducing senescence in myoblasts, detecting aging markers, and performing comprehensive analyses to assess the effects of myoblast aging on different factors.

  • Culture with serum from the elderly. Studies have shown that factors in the serum of aged individuals can influence the aging process in cells. We subject myoblasts to a microenvironment resembling that of an aged individual to mimic the physiological conditions and induce senescence. Our method allows us to help clients investigate the effects of aging factors present in the serum on myoblast behavior, gene expression, and cellular functions.
  • Microgravity induction of myoblasts. We also employ microgravity induction techniques to study the effects of gravity on myoblast aging. Our specialized equipment allows clients to the creation of controlled microgravity conditions, enabling the study of changes in myoblast morphology, gene expression, protein synthesis, and cellular signaling pathways.

Overview

Modeling Methods

Applications

Fig. 1 Effect of senescent microenvironment on MSCs. (Chen H, et al., 2022)

Mesenchymal stem cells (MSCs) are mesoderm-derived progenitor cells that have fibroblast-like morphology, adhere to a tissue culture flask, express a specific set of surface CD markers, and differentiate into osteocytes, adipocytes, and chondrocytes. With aging, a portion of cells, including MSCs, become senescent, and these senescent cells accumulate and promote various age-related diseases

Culturing senescent MSCs provides an opportunity to understand the molecular and cellular changes that occur during the aging process. CD BioSciences offers customization services for culturing senescent MSCs. Our customization services for senescent MSC serve as a valuable tool for drug screening and therapeutic development in the field of aging-related diseases.

  • Long culture passage induction of MSCs. We have developed reliable solutions that involve subjecting MSCs to extended culture periods, allowing them to undergo replicative senescence. During this process, MSCs experience a gradual loss of proliferative capacity and exhibit characteristic senescence-associated phenotypic changes. Through our expertise and culturing techniques, we optimize culture conditions, such as media composition, supplementation of growth factors, and oxygen tension, to promote the development of senescence in MSCs.
  • Microgravity induction of MSCs. Microgravity has been shown to affect cellular behavior and trigger senescence in various cell types. Utilizing bioreactor systems and simulating microgravity environments, CD BioSciences can culture MSCs under conditions that enable ground-based microgravity research. We also support customizing the culture conditions, including the duration of microgravity exposure and optimization of nutrient supply, to provide our clients with senescent MSCs.

The senescent phenotype of MSCs holds great potential for understanding and addressing aging-related diseases. CD BioSciences recognizes the importance of exploring the applications of senescent MSCs in various therapeutic areas.

  • Senescent MSCs in age-related tissue regeneration. We offer customization services for culturing senescent MSCs to help clients investigate the regenerative potential of senescent cells in age-related tissue damage, such as osteoarthritis, neurodegenerative disorders, and cardiovascular diseases.
  • Senescent MSCs in aging-related immunomodulation. Our customization services for culturing senescent MSCs assist clients in studying the impact of senescence on MSC-mediated immunomodulation in aging-related immune disorders, such as autoimmune diseases and chronic inflammation.
  • Senescent MSCs in age-related cancer research. Our clients can employ our senescent MSCs in cancer research to investigate the interactions between senescent MSCs and cancer cells, tumor microenvironment remodeling, and the potential role of senescence-associated secretory phenotype (SASP) in age-related carcinogenesis.

Overview

Modeling Methods

Fig. 1 Four areas of SASP function.(Hoare M, Narita M, 2013)

It has been known that senescence-associated secretory phenotype (SASP) triggers senescence of the surrounding normal cells. SASP involves the secretion of various pro-inflammatory cytokines, chemokines, growth factors, and matrix-remodeling enzymes by senescent cells. This creates an inflammatory microenvironment that can induce senescence in neighboring normal cells through paracrine signaling.

At CD BioSciences, we offer a range of services focused on the customization of paracrine senescent cells. Our services provide researchers with the tools and resources necessary to investigate the effects of senescent cells on neighboring cells in vitro. By utilizing our expertise and techniques, clients can gain valuable insights into the molecular mechanisms, signaling pathways, and therapeutic interventions associated with paracrine cellular senescence.

  • Influenza virus-induced paracrine cellular senescence. Influenza viruses are known to induce cellular senescence in host cells as a defense mechanism. Our team of experts can design and customize in vitro models to mimic this paracrine senescence phenomenon. We initiate the model by infecting the selected cell lines with the influenza virus at an appropriate multiplicity of infection at a specific time point. This step aims to induce viral infection in the host cells, triggering subsequent senescence-related responses. Our services allow clients to investigate the molecular changes and immunomodulatory effects associated with influenza-induced senescence.
  • Stromal cell-induced paracrine cellular senescence. Another area of expertise in our paracrine cellular senescence model customization services revolves around stromal cell-induced senescence. Our team specializes in establishing co-culture systems, where senescent cells and neighboring cells are cultured together. Our services setup mimics the paracrine communication between senescent cells and their surrounding microenvironment. We help clients construct customized in vitro models that incorporate stromal cells and senescent cells.

Customization Services by Cellular Senescence Induction Method

CD BioSciences offers expertise in various cellular senescence induction methods to ensure optimal customization of cellular senescence models. Our clients can choose from a variety of cellular senescence induction methods based on the characteristics and relevance of senescence models to their research goals. By tailoring the cellular senescence induction methods, researchers can gain insights into the molecular mechanisms underlying senescence and its impact on aging-related processes.

Additional Services

We also specialize in developing cellular senescence models based on our client's specific requirements. Our services possess the flexibility to manipulate variables such as cell types, senescence markers, induction protocols, and experimental endpoints.

Cellular Senescence Model Validation Services

Cellular Senescence Model Validation Services

We utilize a panel of senescence markers to confirm the senescent phenotype, including senescence-associated beta-galactosidase (SA-β-gal) staining, p16INK4a expression analysis, and characterization of senescence-associated secretory phenotype (SASP). By employing these reliable markers, we ensure the accuracy and relevance of the senescence models.

Genetic Modification Services in Cellular Senescence Models

Genetic Modification Services in Cellular Senescence Models

We offer the flexibility to incorporate genetic modifications into the cellur senescence models. We utilize advanced gene editing technologies, such as CRISPR-Cas9, to introduce gene knockdown or overexpression. Our customization allows for the investigation of the impact of specific genes on senescence-related processes and the identification of potential therapeutic targets.

Our Service Features

  • Expertise in cellular senescence
  • Diverse cellular senescence induction methods
  • Customization and flexibility
  • Quality control and validation
  • Reliable track records

CD BioSciences offers comprehensive cellular senescence model customization services to facilitate research in the field of aging. By offering a wide range of cell types and cellular senescence induction methods, we ensure that our customers receive customized senescence models that align with their research goals. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

  1. Noren Hooten N, Evans MK. Techniques to Induce and Quantify Cellular Senescence. J Vis Exp. 2017, (123): 55533.
  2. Hwang HJ, et al. Factors and Pathways Modulating Endothelial Cell Senescence in Vascular Aging. Int J Mol Sci, 2022, 23 (17): 10135.
  3. Chen H, et al. Aging and Mesenchymal Stem Cells: Therapeutic Opportunities and Challenges in the Older Group. Gerontology, 2022, 68 (3): 339-352.
  4. Hoare M, Narita M. Transmitting senescence to the cell neighborhood. Nat Cell Biol, 2013, 15 (8): 887-9.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.