In Vitro Cellular Senescence Model Customization Services

At CD BioSciences, we employ various methods to induce senescence in fibroblasts, faithfully recapitulating the aging process in vitro. By simulating the key hallmarks of aging, our customized services enable clients to study the complex interplay of cellular and molecular events associated with aging.

• UVB irradiation of fibroblast. UVB radiation triggers DNA damage and oxidative stress, activating cellular senescence pathways. We utilize controlled UVB irradiation to induce senescence in fibroblasts, contributing to the study of the process of photoaging in the skin. Our approach provides researchers with a valuable tool to investigate the impact of UV radiation on fibroblast aging and study the molecular mechanisms involved.
• Accelerated proliferation of fibroblast. We provide services for constructing senescent fibroblast models by inducing cell proliferation. By culturing fibroblasts with optimized media for rapid cell division, we can induce replicative senescence more efficiently. Our approach allows clients to study the effects of accelerated aging on fibroblast behavior and explore potential interventions to counteract premature aging.
• In vitro H₂O₂ induction. Our services help clients construct the H₂O₂ -induced premature senescence model effectively with appropriate cell-line type, maintaining procedures, dose, time of exposure, and type of solvent. We treat fibroblast cultures with varying concentrations of H2O2 for specific durations to mimic oxidative stress conditions found in aging cells.

• Hydrogen peroxide (H₂O₂) induction. By carefully calibrating the concentration and exposure time of H₂O₂, we can induce controlled levels of oxidative stress to help clients construct aging epithelial cells.
• Exposure to ultraviolet (UV) radiation. Our experts model aging epithelial cells by exposing them to UVA and UVB, which can generate large amounts of reactive oxygen species (ROS) directly.
• Doxorubicin induction of aging epithelial cells. In addition to oxidative stress induction, we also utilize doxorubicin, a chemotherapeutic agent, to induce senescence in epithelial cells. Our experienced team of scientists at CD BioSciences has optimized the doxorubicin induction protocols to ensure reproducibility and minimize cell-to-cell variability. Our services allow for an accurate and reliable assessment of the aging phenotype in cultured epithelial cells.

• Induction of oxidative stress in ECs. At CD BioSciences, we have developed robust approaches to induce oxidative stress in ECs for studying the aging process. We carefully calibrate the concentration and exposure time of H₂O₂ to induce controlled levels of oxidative stress in ECs, which allows for the investigation of the molecular and cellular changes associated with senescence.
• Induction of replicative senescence in ECs. With each cell division, telomeres gradually shorten until a critical threshold is reached, triggering cell cycle arrest and cellular senescence. We offer services for the long-term culture of ECs for induction of replicative senescence. Our approach enables clients to study telomere shortening in aging ECs and their implications in age-related diseases.
• Induction senescence via Inflammatory signals in ECs. Inflammatory cytokine can stimulate the senescence-associated secretory phenotype (SASP) in ECs. This secretory phenotype involves the secretion of various factors that perpetuate senescence and contribute to tissue dysfunction. We offer customized services for exposing ECs to pro-inflammatory signals, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), allowing clients to investigate the molecular mechanisms involved in inflammatory-induced senescence and its implications in age-related diseases.
• Induction of mechanical stress in Ecs. We provide services for subjecting ECs to controlled mechanical stress, such as high fluid shear stress or cyclic stretch. Our approaches can activate signaling pathways related to oxidative stress, inflammation, and mechanotransduction, ultimately leading to endothelial cell senescence.

We employ advanced techniques to induce aging chondrocyte models. By replicating the natural aging process, we can study the cellular changes associated with senescence and develop targeted therapies.

• Irradiation induction of chondrocytes. Exposure to ionizing radiation triggers DNA damage, leading to the activation of cellular senescence pathways. We help clients develop aging chondrocytes with precise and controlled exposure to irradiation, minimizing potential damage and maximizing the senescence induction efficiency. Our expertise in this area enables us to provide reliable and reproducible aging chondrocyte models for aging research and drug development.
• Pro-inflammatory cytokine induction of chondrocytes. We utilize pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α), to induce senescence in chondrocytes and stimulate cellular responses that mimic the inflammatory microenvironment observed in aging joints. Our services assist clients in investigating the molecular mechanisms underlying senescence-related changes in cartilage.
• Isolation from damaged cartilage to induce senescence in chondrocytes. We also offer a unique approach to inducing senescence in chondrocytes by isolating cells from damaged cartilage. In damaged joints, chondrocytes experience increased stress and mechanical loading, leading to accelerated senescence. By isolating these aging chondrocytes, our services enable clients to study their distinct characteristics and explore potential interventions to alleviate the aging-related effects on cartilage.

• Culture with serum from the elderly. Studies have shown that factors in the serum of aged individuals can influence the aging process in cells. We subject myoblasts to a microenvironment resembling that of an aged individual to mimic the physiological conditions and induce senescence. Our method allows us to help clients investigate the effects of aging factors present in the serum on myoblast behavior, gene expression, and cellular functions.
• Microgravity induction of myoblasts. We also employ microgravity induction techniques to study the effects of gravity on myoblast aging. Our specialized equipment allows clients to the creation of controlled microgravity conditions, enabling the study of changes in myoblast morphology, gene expression, protein synthesis, and cellular signaling pathways.

• Long culture passage induction of MSCs. We have developed reliable solutions that involve subjecting MSCs to extended culture periods, allowing them to undergo replicative senescence. During this process, MSCs experience a gradual loss of proliferative capacity and exhibit characteristic senescence-associated phenotypic changes. Through our expertise and culturing techniques, we optimize culture conditions, such as media composition, supplementation of growth factors, and oxygen tension, to promote the development of senescence in MSCs.
• Microgravity induction of MSCs. Microgravity has been shown to affect cellular behavior and trigger senescence in various cell types. Utilizing bioreactor systems and simulating microgravity environments, CD BioSciences can culture MSCs under conditions that enable ground-based microgravity research. We also support customizing the culture conditions, including the duration of microgravity exposure and optimization of nutrient supply, to provide our clients with senescent MSCs.

The senescent phenotype of MSCs holds great potential for understanding and addressing aging-related diseases. CD BioSciences recognizes the importance of exploring the applications of senescent MSCs in various therapeutic areas.

• Senescent MSCs in age-related tissue regeneration. We offer customization services for culturing senescent MSCs to help clients investigate the regenerative potential of senescent cells in age-related tissue damage, such as osteoarthritis, neurodegenerative disorders, and cardiovascular diseases.
• Senescent MSCs in aging-related immunomodulation. Our customization services for culturing senescent MSCs assist clients in studying the impact of senescence on MSC-mediated immunomodulation in aging-related immune disorders, such as autoimmune diseases and chronic inflammation.
• Senescent MSCs in age-related cancer research. Our clients can employ our senescent MSCs in cancer research to investigate the interactions between senescent MSCs and cancer cells, tumor microenvironment remodeling, and the potential role of senescence-associated secretory phenotype (SASP) in age-related carcinogenesis.

• Influenza virus-induced paracrine cellular senescence. Influenza viruses are known to induce cellular senescence in host cells as a defense mechanism. Our team of experts can design and customize in vitro models to mimic this paracrine senescence phenomenon. We initiate the model by infecting the selected cell lines with the influenza virus at an appropriate multiplicity of infection at a specific time point. This step aims to induce viral infection in the host cells, triggering subsequent senescence-related responses. Our services allow clients to investigate the molecular changes and immunomodulatory effects associated with influenza-induced senescence.
• Stromal cell-induced paracrine cellular senescence. Another area of expertise in our paracrine cellular senescence model customization services revolves around stromal cell-induced senescence. Our team specializes in establishing co-culture systems, where senescent cells and neighboring cells are cultured together. Our services setup mimics the paracrine communication between senescent cells and their surrounding microenvironment. We help clients construct customized in vitro models that incorporate stromal cells and senescent cells.