Cellular senescence is a complex biological process characterized by permanent growth arrest and a distinct senescence-associated phenotype. It plays a crucial role in aging and aging-related diseases. Cellular senescence models are experimental systems in which cells are induced to undergo senescence through various methods. These models enable researchers to investigate the molecular mechanisms, functional changes, and phenotypic characteristics associated with senescence. Customization of this model involves a targeted approach to address key questions and explore innovative strategies in the field of aging research.
CD BioSciences is committed to delivering top-notch, customized services for cellular senescence models according to different cell types and cellular senescence induction methods, empowering our clients to advance their aging research and therapeutic discovery endeavors with precision and scientific excellence. We also specialize in developing cellular senescence models based on our client's specific requirements. Our services possess the flexibility to manipulate variables such as cell types, senescence markers, induction protocols, and experimental endpoints.
At CD BioSciences, we understand that different cell types exhibit unique characteristics and responses to cellular senescence induction stimuli. Our team of experts offers guidance on the advantages and limitations of each cell type to help researchers choose the appropriate model for their specific research goals. We employ a variety of well-established methods to induce cellular senescence, such as replicative exhaustion, genotoxic stress, oxidative stress, and oncogene activation. By customizing the senescence models based on cell type, researchers can gain insights into tissue-specific senescence mechanisms and address the relevance of senescence in various physiological and pathological contexts. We offer customized services of senescence models for a wide range of cell types, including but not limited to the following.
Senescent-Induced Fibroblasts
Senescent-Induced Epithelial Cells
Senescent-Induced Endothelial Cells
Senescent-Induced Chondrocytes
Senescent-Induced Myoblasts
Senescent-Induced Mesenchymal Stem Cells
Paracrine Senescent Cells
Overview
Modeling Methods
Fig. 1 Schematic illustration of senescent fibroblasts. (Noren Hooten N and Evans MK, 2017)
The development of therapeutic approaches to slow or reverse the adverse physiological and pathological changes associated with aging has been considered an important goal of gerontological research. As cellular senescence has been characterized as the basis of aging in organisms, the culture and subculture of normal human diploid fibroblasts to mimic in vivo aging processes has been developed as an important method to study the molecular events involved in aging.
CD BioSciences, a pioneer in aging research, offers a range of customization services for senescent fibroblasts, providing our clients with valuable tools to unravel the mechanisms underlying aging and explore potential anti-aging strategies.
At CD BioSciences, we employ various methods to induce senescence in fibroblasts, faithfully recapitulating the aging process in vitro. By simulating the key hallmarks of aging, our customized services enable clients to study the complex interplay of cellular and molecular events associated with aging.
Overview
Modeling Methods
Epithelial cells play a critical role in maintaining the integrity and function of various tissues and organs in the body. As these cells age, their functionality and regenerative capacity decline, leading to impaired tissue homeostasis and increased susceptibility to disease. Modeling senescence in epithelial cells is of paramount importance for understanding the underlying mechanisms and developing targeted interventions to promote healthy aging and prevent age-related diseases.
CD BioSciences is a leading company specializing in providing high-quality customization services for aging epithelial cells. With years of experience in the industry, we have developed a deep understanding of the unique challenges and requirements associated with studying aging in epithelial cells.
Overview
Modeling Methods
Fig. 1 Characteristics of senescent endothelial cells. (Hwang HJ, et al., 2022)
The endothelial cell (EC) monolayer forms the inner cellular lining of all blood vessels forming a critical interface between blood and tissue. Vascular endothelium is involved in physiological functions, which include regulation of blood fluidity, hemostasis and clotting, vascular tone, immune responses, inflammation, angiogenesis, and metabolism. Modeling aging ECs mimics the aging process in a controlled laboratory setting, enabling researchers to study age-related changes without the confounding factors present in in vivo models.
CD BioSciences is a leading company specializing in providing customization services for aging ECs. We offer tailored solutions to meet the unique research needs of scientists and researchers studying age-related diseases and the cellular mechanisms underlying EC senescence.
Overview
Modeling Methods
Chondrocytes play a vital role in maintaining the structure and function of cartilage, which is crucial for joint health and mobility. As chondrocytes age, they experience changes in their morphology, functionality, and gene expression patterns, which can significantly impact the health and integrity of the surrounding cartilage tissue. Culturing aging chondrocytes enables researchers to study the effects of aging on the mechanical behavior and integrity of cartilage, contributing to a better understanding of age-related joint degeneration.
CD BioSciences is a leading company specializing in the field of longevity research and offers services in the customization of aging chondrocytes.
We employ advanced techniques to induce aging chondrocyte models. By replicating the natural aging process, we can study the cellular changes associated with senescence and develop targeted therapies.
Overview
Modeling Methods
Myogenesis gradually deteriorates as the skeletal muscle ages, contributing to muscle mass loss. Indeed, one of the most serious consequences of aging is the progressive loss of muscle mass (sarcopenia) and function, which affects the quality of life and, ultimately, the survival of elderly people.
CD BioSciences specializes in providing a range of services for aging myoblasts. Our expertise lies in inducing senescence in myoblasts, detecting aging markers, and performing comprehensive analyses to assess the effects of myoblast aging on different factors.
Overview
Modeling Methods
Applications
Fig. 1 Effect of senescent microenvironment on MSCs. (Chen H, et al., 2022)
Mesenchymal stem cells (MSCs) are mesoderm-derived progenitor cells that have fibroblast-like morphology, adhere to a tissue culture flask, express a specific set of surface CD markers, and differentiate into osteocytes, adipocytes, and chondrocytes. With aging, a portion of cells, including MSCs, become senescent, and these senescent cells accumulate and promote various age-related diseases
Culturing senescent MSCs provides an opportunity to understand the molecular and cellular changes that occur during the aging process. CD BioSciences offers customization services for culturing senescent MSCs. Our customization services for senescent MSC serve as a valuable tool for drug screening and therapeutic development in the field of aging-related diseases.
The senescent phenotype of MSCs holds great potential for understanding and addressing aging-related diseases. CD BioSciences recognizes the importance of exploring the applications of senescent MSCs in various therapeutic areas.
Overview
Modeling Methods
Fig. 1 Four areas of SASP function.(Hoare M, Narita M, 2013)
It has been known that senescence-associated secretory phenotype (SASP) triggers senescence of the surrounding normal cells. SASP involves the secretion of various pro-inflammatory cytokines, chemokines, growth factors, and matrix-remodeling enzymes by senescent cells. This creates an inflammatory microenvironment that can induce senescence in neighboring normal cells through paracrine signaling.
At CD BioSciences, we offer a range of services focused on the customization of paracrine senescent cells. Our services provide researchers with the tools and resources necessary to investigate the effects of senescent cells on neighboring cells in vitro. By utilizing our expertise and techniques, clients can gain valuable insights into the molecular mechanisms, signaling pathways, and therapeutic interventions associated with paracrine cellular senescence.
CD BioSciences offers expertise in various cellular senescence induction methods to ensure optimal customization of cellular senescence models. Our clients can choose from a variety of cellular senescence induction methods based on the characteristics and relevance of senescence models to their research goals. By tailoring the cellular senescence induction methods, researchers can gain insights into the molecular mechanisms underlying senescence and its impact on aging-related processes.
We also specialize in developing cellular senescence models based on our client's specific requirements. Our services possess the flexibility to manipulate variables such as cell types, senescence markers, induction protocols, and experimental endpoints.
We utilize a panel of senescence markers to confirm the senescent phenotype, including senescence-associated beta-galactosidase (SA-β-gal) staining, p16INK4a expression analysis, and characterization of senescence-associated secretory phenotype (SASP). By employing these reliable markers, we ensure the accuracy and relevance of the senescence models.
We offer the flexibility to incorporate genetic modifications into the cellur senescence models. We utilize advanced gene editing technologies, such as CRISPR-Cas9, to introduce gene knockdown or overexpression. Our customization allows for the investigation of the impact of specific genes on senescence-related processes and the identification of potential therapeutic targets.
CD BioSciences offers comprehensive cellular senescence model customization services to facilitate research in the field of aging. By offering a wide range of cell types and cellular senescence induction methods, we ensure that our customers receive customized senescence models that align with their research goals. If you are interested in our services, please feel free to contact us or make an online inquiry.
References
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.