Chemotherapy-Induced Cellular Senescence Model Customization Services

Chemotherapy can target cancer cells for destruction or management. The process triggers senescence as a reaction to chemotherapy treatment known as chemotherapy-induced senescence (CIS), which manifests as permanent growth cessation with unique phenotypic and molecular alterations. Most genotoxic treatments trigger a senescence arrest in epithelial cells when they reach appropriate concentrations.

CD BioSciences offers model customization solutions for chemotherapy-induced senescence that support essential research in basic science and pharmaceutical development.

Our Chemotherapy-Induced Cellular Senescence Model Customization Options

Selection of chemotherapeutic agents

At CD BioSciences, we support various chemotherapeutic agents to induce cellular senescence, such as doxorubicin, cisplatin, and etoposide. We consider factors such as the agents' ability to induce senescence, their specificity towards cancer cells, and their overall cytotoxicity. By carefully selecting the most suitable chemotherapeutic agents, we ensure that the chemotherapy-induced cellular senescence model accurately reflects the desired cellular response.

Optimization of concentrations

Too low a concentration may not induce a significant senescent response, while excessively high concentrations can lead to cell death rather than senescence. We utilize extensive experience and knowledge to optimize the concentrations of chemotherapeutic agents to achieve the desired level of cellular senescence.

Determining exposure time

Considering factors such as the kinetics of drug action, the half-life of the agents, and the desired senescence induction timeline, we tailor the exposure time of the chemotherapy drugs to reflect the desired senescent phenotype.

How Do We Construct Chemotherapy-Induced Cellular Senescence Models?

• Experimental design and consultation. Our team of experts collaborates closely with clients to understand their research objectives and experimental requirements. Leveraging our vast experience, we provide reliable experimental design services.
• Cell line selection. Based on the research goals, we assist clients in choosing suitable cell lines. We consider factors such as genetic background and availability to ensure optimal model selection.
• Cellular senescence induction. Using advanced techniques and protocols, we induce cellular senescence in the selected models. Our services involve the precise administration of chemotherapy agents ensuring the viability and integrity of the cells.
• Senescence phenotypic characterization. We perform comprehensive phenotypic characterization to validate the senescent state, including assessing senescence-associated markers, such as senescence-associated beta-galactosidase (SA-β-gal) activity, cell cycle arrest, and alterations in gene expression profiles.

Mechanism Analysis Services for CIS

We provide comprehensive mechanism analysis services to unravel the intricate pathways and signaling networks involved in this phenomenon. Our team of experts utilizes a range of cutting-edge techniques, including genomics, proteomics, and metabolomics, to investigate the molecular changes associated with chemotherapy-induced cellular senescence. By employing high-throughput screening and data analysis, we can identify key regulatory factors, signaling pathways, and potential therapeutic targets.

CD BioSciences is dedicated to providing exceptional services in the field of chemotherapy-induced cellular senescence. Our well-defined workflow ensures accurate and consistent construction of chemotherapy-induced cellular senescence models, from experimental design to senescence phenotypic characterization. If you are interested in our services, please feel free to contact us or make an online inquiry.