Calorie Restriction Mimetic Discovery Services

Caloric restriction (CR) has been shown in numerous studies to extend lifespan and delay the onset of age-related diseases in organisms ranging from yeast to primates. This phenomenon has sparked interest in caloric restriction mimetics (CRMs)—compounds that can mimic the biochemical effects of caloric restriction. Research indicates that CRMs can activate similar pathways in caloric restriction, such as the sirtuin pathway and AMP-activated protein kinase (AMPK). These pathways are critical in regulating metabolism, inflammation, and cellular repair processes.

Fig. 1 General properties of CRMs. (Madeo F, et al., 2014)

The discovery of CRMs has the potential to replicate the benefits of caloric restriction without the need for actual dietary changes. CD BioSciences offers a comprehensive suite of services to facilitate each stage of CRM discovery.

How Do We Develop Calorie Restriction Mimetics as Anti-Aging Drugs?

CRM screening

We can access diverse drug libraries, including natural products and synthetic drugs. Our services help clients efficiently accelerate the identification of promising CRM candidates for further research. We screen targets key pathways associated with aging, such as the sirtuin and mechanistic target of rapamycin (mTOR) pathways, which are critical in regulating cellular homeostasis and longevity. Our high-throughput high-content screening (HTHCS) services focus on screening for specific candidates that can enhance autophagic flux by targeting the deacetylation of cellular proteins, all while ensuring that these candidates do not induce cytotoxicity.

In vitro evaluation of CRMs

We help clients measure autophagic flux to evaluate the biological activity of CRM candidates. Our team of experts also assesses whether CRMs promote cell survival comparing treated cells to controls. Our services can be tailored to meet specific client needs, allowing clients to explore the effects of CRMs on different cell types, including stem cells, neuron-like cells, and muscle cells.

In vivo evaluation of CRMs

We utilize various animal models, such as mouse, yeast, and Drosophila models, to study the pharmacokinetics and pharmacodynamics of CRM candidates. We also perform lifespan studies in various model organisms to determine if mimetics can extend the lifespan under controlled conditions. Our in vivo studies include a range of assessments, such as metabolic profiling, cognitive function tests, and physical performance evaluations.

Analysis of the Role of Calorie Restriction Mimetics in Aging-Related Metabolic Disease

CRMs hold significant promise in aging-related metabolic diseases, such as obesity, type 2 diabetes, and cardiovascular disorders. We help clients assess metabolic changes, including insulin sensitivity, lipid profiles, and energy metabolism, to determine the impact of CRMs. By studying the metabolome, we can identify the downstream effects of mimetic treatment on protein abundance and metabolic pathways.

CD BioSciences offers a novel and promising way to identify and characterize drugs that mimic the beneficial effects of caloric restriction. Our team provides statistical analysis to help clients make informed decisions during the drug development process. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. Nassar K, et al. The significance of caloric restriction mimetics as anti-aging drugs. Biochem Biophys Res Commun, 2024, 692: 149354.
2. Madeo F, et al. Caloric restriction mimetics: towards a molecular definition. Nat Rev Drug Discov, 2014, 13 (10): 727-40.