Aging describes the multifaceted decline of cellular and organismal function over time and represents a major risk factor for susceptibility to diseases. Yeast, specifically Saccharomyces cerevisiae, is a widely used model organism in aging research due to its genetic tractability, short lifespan, and evolutionary conservation of aging-related genes and pathways. In yeast, many pathways relevant to aging and disease in humans are well conserved, including nutrient signaling, cell cycle regulation, DNA repair mechanisms, mitochondrial homeostasis, protein folding and secretion, proteostasis, stress response, and regulated cell death.
Fig. 1 Conserved hallmarks of aging between yeast and humans. (Dahiya R, et al,, 2020)
At CD BioSciences, we provide yeast aging model customization services as tailored tools, aimed to address specific research questions related to aging biology. Our team of skilled scientists and technicians possesses extensive experience in yeast biology and aging research and is dedicated to delivering high-quality results.
We offer an extensive collection of yeast strains with various genetic backgrounds and characteristics, such as S. cerevisae and the fission yeast Schizosaccharomyces pombe. Our expert team works closely with clients to select or develop yeast strains suitable for their specific research goals.
Through control of culturing conditions, we provide yeast replicative and chronological aging models. In yeast replicative aging models, we use micromanipulation techniques to physically separate mother cells from their daughter cells during cell division, tracking the number of divisions each mother cell undergoes. For chronological aging models, we culture yeast cells in nutrient-rich media until they reach the stationary phase, where they exit the cell cycle and enter a non-dividing, quiescent state. Over time, these non-dividing cells accumulate damage and exhibit characteristics of chronological aging.
Longevity assays are essential for measuring the lifespan of yeast cells and evaluating the effects of genetic or environmental interventions on aging. We offer two types of longevity assays, replicative lifespan (RLS) and chronological lifespan (CLS) assays.
| Assays | Service details |
| RLS assay | RLS assay measures the number of times a mother yeast cell can divide and produce daughter cells before senescence. We utilize advanced microscopy techniques and automated image analysis to accurately track and quantify the division events, enabling precise measurements of RLS. |
| CLS assay | CLS assay measures the survival of yeast cells in a non-dividing state, reflecting the aging of post-mitotic cells. We offer robust protocols for monitoring CLS, including cell viability measurements, cell cycle analysis, and assessment of specific aging-related markers. |
We offer advanced high-throughput analysis services to monitor yeast aging, providing our clients with efficient and reliable data for their studies. Specifically, we utilize microfluidic platforms to help clients track key parameters of yeast aging, such as cell division, growth rate, and lifespan, at the single-cell level.
The versatile nature of yeast allows researchers to unravel the molecular mechanisms underlying aging and identify conserved pathways and genes involved in the aging process. The insights from yeast studies can directly translate to higher organisms, including humans, uncovering potential therapeutic targets and strategies for healthy aging.
We use yeast models for detailed mechanistic investigations into aging-related processes. Utilizing our advanced techniques, such as transcriptomics, proteomics, and metabolomics, our clients can dissect the molecular networks and metabolic changes contributing to aging and aging-related diseases.
Based on yeast models, our services offer a cost-effective and efficient drug discovery and screening system. High-throughput screening using yeast models allows for the identification of compounds with potential anti-aging properties, providing a stepping stone for further development and testing.
Yeast models have emerged as indispensable tools in aging research, allowing scientists to unravel the intricate mechanisms underlying aging and age-related diseases. CD BioSciences, with our extensive experience and expertise, offers comprehensive yeast model customization services for aging research. If you are interested in our services, please feel free to contact us or make an online inquiry.
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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
CD BioScience is a contract research organization focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
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