CAT. NO.: AC-02008
General Info | |
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Size | 50 tests/200 tests |
Product Overview | This product enables the rapid separation and purification of genomic DNA from 200-400 μL of fresh or frozen human or animal whole blood in just 15-20 minutes. The product does not require the use of proteinase K. After the whole blood is dissolved by Solution BFL1, the hemoglobin is removed by Solution BFL2 precipitation. The genomic DNA in the supernatant can be bound to the purification column. Subsequent washing with Buffer RP and Wash Buffer removes residual proteins on the membrane and PCR inhibitors. The genomic DNA is eluted by Elution Buffer and can be used in a wide range of molecular biology experiments immediately. |
Storage Conditions | Room temperature. |
Notes | 1. The sample should be avoided from repeated freeze-thaw cycles as it can result in smaller DNA fragments and decreased extraction yield. 2. If a precipitate is observed in Solution BFL1 or Solution BFL2, it can be re-dissolved by placing it in a 56°C water bath. After re-dissolving, shake the solution and it will be ready for use. 3. All centrifugation steps should be performed using a benchtop centrifuge at room temperature. |
Product Details | |
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Samples | Whole Blood |
Components | Solution BFL1 Solution BFL2 Buffer RP Wash Buffer Elution Buffer Adsorption Column |
Highlights | 1. Fast and low toxicity 2. Wide applicability 3. High quality 4. Good repeatability and high output |
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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.