Telomerase Colorimetric Cell-Based ELISA Kit

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CAT. NO.: TK068

General Info
Size	1×96 Wells / 5×96 Wells
Product Overview	This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase (TERT) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TERT. The ELISA analytical biochemical technique of the Kit is based on TERT antibody-TERT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TERT antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TERT.
Storage Conditions	Store at 4°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Human
Shipping Conditions	Dry Ice

Product Details
Assay Type	Non-Phospho Cell-Based ELISA
Samples	Researchers plate their cell line of choice
Sensitivity	>=5000 cells/well
Detection Wavelength	450 nm

Target Info
Molecular Weight	126,997 Da
NCBI Accession	NP_001180305.1
NCBI GenBank Nucleotide	NM_001193376.1
NCBI GeneID	7015
NCBI Official Full Name	telomerase reverse transcriptase isoform 2
NCBI Official Symbol	TERT
NCBI Official Synonym Full Names	telomerase reverse transcriptase
NCBI Official Synonym Symbols	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
NCBI Protein Information	telomerase reverse transcriptase
OMIM	AF018167 mRNA
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Gene Names	EST2, TCS1, TRT, TP2
UniProt Synonym Protein Names	HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Protein Family	Telomerase component
NCBI Summary	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
UniProt Comments	TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: DNA-binding; EC 2.7.7.49; Nucleolus; TransferaseChromosomal Location of Human Ortholog: 5p15.33Cellular Component: chromosome, telomeric region; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus; plasma membrane; PML body; telomerase catalytic core complex; telomerase holoenzyme complex; TERT-RMRP complexMolecular Function: chaperone binding; DNA binding; identical protein binding; metal ion binding; nucleotidyltransferase activity; protein binding; protein C-terminus binding; protein homodimerization activity; protein N-terminus binding; RNA binding; RNA-directed DNA polymerase activity; RNA-directed RNA polymerase activity; telomerase activity; telomerase RNA binding; telomeric DNA binding; telomeric template RNA reverse transcriptase activity; template-free RNA nucleotidyltransferase; tRNA bindingBiological Process: beta-catenin-TCF complex assembly; cellular response to hypoxia; DNA biosynthetic process; DNA strand elongation; mitochondrion organization; negative regulation of cellular senescence; negative regulation of endothelial cell apoptotic process; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of gene expression; negative regulation of neuron apoptosis; positive regulation of angiogenesis; positive regulation of G1/S transition of mitotic cell cycle; positive regulation of glucose import; positive regulation of hair cycle; positive regulation of nitric-oxide synthase activity; positive regulation of protein binding; positive regulation of protein localization to nucleolus; positive regulation of stem cell proliferation; positive regulation of vascular associated smooth muscle cell migration; positive regulation of vascular smooth muscle cell proliferation; positive regulation of Wnt receptor signaling pathway; regulation of protein stability; replicative senescence; response to cadmium ion; RNA biosynthetic process; RNA interference, production of siRNA; RNA-dependent DNA replication; telomere maintenance; telomere maintenance via telomeraseDisease: Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1; Pulmonary Fibrosis, Idiopathic

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