Single-Cell Transcriptome Profiling for Aging Characterization

Single-cell RNA sequencing (scRNA-seq) measures the expression levels of thousands of genes simultaneously. It has emerged as a powerful tool to unravel the molecular landscape of aging at the cellular level. At CD BioSciences, we offer cutting-edge single-cell transcriptome profiling services that enable our clients to gain unprecedented insights into the gene expression dynamics of aging cells.

Technology Platforms for Single-Cell Transcriptome Sequencing

Our primary scRNA-seq platforms include droplet-based and plate-based single-cell RNA sequencing (scRNA-seq). These platforms offer distinct advantages in terms of scalability, sensitivity, and throughput. In droplet-based scRNA-seq, we can capture individual cells in nanoliter-scale droplets to obtain high-resolution gene expression profiles. In plate-based scRNA-seq, we isolate individual cells into wells of a microtiter plate and perform high-throughput sequencing to quantify gene expression in each cell.

Our Workflow for Single-Cell Transcriptome Sequencing

• Sample collection and preparation
  We establish the optimal sample collection and preparation protocols for our client's specific aging research projects. Our previous successful biospecimen types include healthy aging cohorts, aging-related disease cohorts, aging animal models, etc.
• Cell isolation and capture
  Depending on the sample type and experimental requirements, we employ various techniques such as fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), or manual cell picking to isolate specific cell populations of interest. Once isolated, the cells are captured using droplet-based or plate-based methods.
• RNA extraction, library preparation, sequencing
  After cell capture, we perform RNA extraction to isolate the cellular RNA. The extracted RNA is then subjected to reverse transcription to convert it into cDNA for library construction and high-throughput sequencing.
• Data analysis
  We perform quality control, read alignment, gene quantification, normalization, and statistical analysis to identify differentially expressed genes, define cell clusters, reconstruct gene regulatory networks, and detect aging-related genes.

Applications of Single-Cell Transcriptome Profiling in Aging Studies

Characterization of cell-type specific aging

By profiling gene expression in individual cells, we can identify cell-type-specific gene expression changes associated with aging. This helps us understand how different cell types contribute to the aging process.

Evaluation of interventions

We also use single-cell transcriptome sequencing to assess the effects of potential interventions aimed at promoting healthy aging. By comparing gene expression profiles before and after intervention, we can evaluate the impact of therapeutic strategies on cellular gene expression patterns.

CD BioSciences, with its single-cell transcriptome profiling technology platforms, rigorous workflow, and data analysis expertise, helps our clients gain valuable insights into the molecular changes associated with aging. If you are interested in our services, please feel free to contact us or make an online inquiry.