Single-Cell Metabolomic Profiling of Aging

Single-cell metabolomics aims to elucidate tissue heterogeneity of the metabolome to reveal the cell types responsible for alterations in metabolite profiling. This approach provides valuable insights into metabolic alterations that occur at the cellular level during aging, which can help understand underlying mechanisms and identify potential targets for interventions.

Fig. 1 Single-cell metabolomics based on mass spectrometry. (Liu QL, et al., 2023)

CD BioSciences offers single-cell metabolomics services that enable in-depth analysis of aging-related processes at the cellular level. We are committed to helping our customers advance their scientific research by revealing the complex metabolic changes that occur at the cellular level during the aging process.

Overview of Our Single-Cell Metabolomics Services

Single-cell metabolite profiling of aging

We provide a comprehensive analysis of metabolites within individual cells to identify changes associated with aging. This could include the measurement of key metabolites such as energy-related molecules (e.g., ATP, NADH), amino acids, and lipids.

Fluxomics analysis of aging

We focus on determining the metabolic fluxes within individual cells, shedding light on changes in cellular metabolic pathways that occur during aging. Our service aims to reveal alterations in cellular energy metabolism and other key biochemical processes.

Metabolic pathway analysis of aging

We offer an in-depth analysis of specific metabolic pathways known to be implicated in aging, such as mitochondrial metabolism, lipid metabolism, or oxidative stress-related pathways, enabling our clients to identify specific metabolic dysregulations associated with aging.

Technology Platforms for Single-Cell Metabolomics

Our company leverages various technology platforms to perform single-cell metabolomic profiling. Among all detection methods, MS-based techniques stand out, mainly including electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), and secondary ion mass spectrometry (SIMS) techniques. Our instruments are equipped with high-resolution capabilities, enabling the identification and quantification of a wide range of metabolites.

Our Workflow for Single-Cell Metabolomics

• Sample collection
  We collect samples from age-stratified populations, such as young, middle-aged, and elderly individuals, which can help in comparing the metabolic profiles of cells at different stages of the aging process. If the research involves senescence in the context of age-related diseases, we also collect biological samples from diseased tissues relevant to conditions such as neurodegenerative diseases or age-related metabolic disorders.
• Cell sorting and quenching
  After sample collection, CD BioSciences employs advanced cell sorting techniques to isolate specific cell populations of interest. This step is essential to eliminate unwanted cellular heterogeneity and obtain pure cell populations for metabolomic analysis. Following cell sorting, rapid quenching methods are employed to halt metabolic activity and preserve the cellular metabolome.
• Data acquisition and analysis
  CD BioSciences utilizes cutting-edge mass spectrometry platforms to acquire metabolomic data from single cells. Our team of skilled experts applies statistical methods, pathway analysis, and machine learning algorithms to identify significant metabolic changes associated with aging and to elucidate underlying metabolic pathways.

At CD BioSciences, our team of highly skilled scientists possesses extensive expertise in single-cell metabolomics and aging research. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Liu QL, et al. Recent advances in single-cell metabolomics based on mass spectrometry. CCS Chemistry, 2023, 5 (2): 310-324.