CAT. NO.: TK067
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Telomerase Reverse Transcriptase (TERT) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing TERT. The ELISA analytical biochemical technique of the Kit is based on TERT antibody-TERT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TERT antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant TERT. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Sheep |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Samples | Serum, Plasma, Cell Culture Supernatants, Body Fluid, Tissue Homogenate |
| Sample Preparation | We recommend preliminary experimentation using undiluted samples or dilutions at ratios of 1:2 or 1:4. Please refrain from exceeding a 1:10 dilution, as it surpasses the prescribed dilution threshold for this kit. |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 88,965 Da |
| NCBI Accession | O14746.1 |
| NCBI GeneID | 7015 |
| NCBI Official Full Name | Telomerase reverse transcriptase |
| NCBI Official Symbol | TERT |
| NCBI Official Synonym Full Names | telomerase reverse transcriptase |
| NCBI Official Synonym Symbols | TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1 |
| NCBI Protein Information | telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2 |
| OMIM | 615134 |
| UniProt Entry Name | TERT_HUMAN |
| UniProt Gene Name | TERT |
| UniProt Protein Name | Telomerase reverse transcriptase |
| UniProt Synonym Gene Names | EST2, TCS1, TRT, TP2 |
| UniProt Synonym Protein Names | HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2 |
| UniProt Primary Accession | O14746 |
| UniProt Secondary Accession | O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46 |
| Protein Family | Telomerase reverse transcriptase |
| NCBI Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Comments | TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: EC 2.7.7.49; Transferase; Nucleolus; DNA-bindingChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA bindingBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; RNA interference, production of siRNA; telomere maintenance; positive regulation of Wnt receptor signaling pathwayDisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1 |
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