CAT. NO.: SMT002
| General Info | |
|---|---|
| Size | 100 tests |
| Product Overview | Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. This product mainly uses relative quantitative qPCR to directly compare the average telomere length of the sample, that is, the copy number ratio (T/S) of the telomere repeat sequence (Tel) and the genome single copy gene (SCR) copy number (T/S) is used as the telomere relative length. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies the 100 bp-long regions on human chromosome 17 and serves as a reference for data normalization. |
| Storage Conditions | Store the 2×qPCR master mix at -20°C, primers at -20°C, and nuclease-free H2O at 4°C, protected from light. |
| Notes | The 2×qPCR master mix contains SYBR Green as the reporter dye and does NOT contain a ROX passive reference dye. If the qPCR instrument being used has a "ROX passive reference dye" option, please deselect this option. |
| Reactivity | Human |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | qPCR |
| Samples | Genomic DNA |
| Components | 2×qPCR master mix Telomere primer set, lyophilized SCR primer set, lyophilized Nuclease-free H2O |
| Highlights | 1. The carefully designed primers ensure high efficiency for trustworthy quantification and no nonspecific amplification. 2. The kit includes the 2×qPCR Master Mix, which contains SYBR Green, dNTPs, thermally activated Taq DNA polymerase, and inert gold-color loading indicator. Taq DNA polymerase can effectively prevent the formation of unnecessary primer dimers. The gold-color loading indicator allows easy visualization and tracking of sample loading in qPCR plates or tubes. |
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