Reduced Representation Bisulfite Sequencing for Aging Studies

In aging research, due to the time and cost involved in sequencing and bioinformatics for genome-wide assays, many researchers have turned to a method called reduced-representation bisulfite sequencing (RRBS) for genome-wide DNA methylation assays. CD BioSciences, a leading biological research company, offers comprehensive services for RRBS in aging studies.

Overview of Our Reduced Representation Bisulfite Sequencing Services

For RRBS, CD BioSciences uses restriction enzyme digestion and DNA size selection to focus the analysis on a subset of the genome where the majority of the DNA methylation occurs.

DNA methylation profiling for aging research

With RRBS, we can analyze the methylation status of CpG dinucleotides in a subset of genomic regions, typically regions rich in CpG islands and gene regulatory regions. So, we help our clients generate comprehensive DNA methylation profiles and identify age-related DNA methylation changes in these specific regions.

Identification of differentially methylated CpG sites

By comparing RRBS data between different age groups, we can identify individual CpG sites that display significant age-related differential methylation. This information can help our clients understand the specific CpG sites that undergo methylation changes during aging.

Epigenetic regulation analysis

Using RRBS data, we can provide insights into the epigenetic regulation patterns associated with aging. By analyzing the methylation patterns in specific genomic regions, such as promoters, enhancers, and gene bodies, we can determine how these regions are epigenetically regulated during the aging process.

Guidelines for Sample Preparation

Accurate and reliable sample preparation is essential for obtaining meaningful results in RRBS. At CD BioSciences, we follow guidelines to ensure the quality of samples used in aging studies.

• Sample selection
  Our experts recommend using a diverse set of biological samples, including different tissues and cell types relevant to aging. This ensures a comprehensive understanding of the epigenetic changes associated with aging. Our services also support biological samples in aging animal models, such as mice and fish.
• Sample size
  Adequate sample size is crucial for statistical power in aging studies. We guide sample size determination based on the specific research objectives and expected effect sizes.

Our Workflow of Reduced Representation Bisulfite Sequencing Services

Fig. 1 General principles of RRBS.

Our workflow encompasses the entire process, including DNA extraction and digestion, end repair and adapter ligation, size selection, bisulfite conversion, PCR amplification, next-generation sequencing, and bioinformatics. We employ bisulfite conversion to deaminate non-methylated cytosines, converting them to uracil residues that will ultimately be read as thymidines in the analysis. Methylated cytosines are protected from bisulfite conversion and are therefore still read as cytosines in the analysis.

After sequencing, we use specialized methylation analysis tools to identify and quantify DNA methylation levels at individual CpG sites. By comparing DNA methylation patterns between different age groups, we can identify regions that show age-related changes in DNA methylation.

With advanced sequencing platforms and bioinformatics tools, CD BioSciences can offer high-quality RRBS services with accurate sequencing results and in-depth data analysis. If you are interested in our services, please feel free to contact us or make an online inquiry.