CAT. NO.: TK131
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This sandwich Kit is designed for the precise quantitative detection of Rat Telomere Repeat Binding Factor 2 (also known as TRF2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. This Kit utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) method. The plate has been pre-coated with Rat TRF2 antibody. TRF2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRF2 Antibody is added and binds to TRF2 in the sample. Following this, Streptavidin-HRP is introduced and binds to the Biotinylated TRF2 antibody. During a washing step, any unbound Streptavidin-HRP is removed. A substrate solution is then added, and color development occurs in proportion to the quantity of Rat TRF2. The reaction is halted by adding an acidic stop solution, and the absorbance is measured at 450 nm. |
| Storage Conditions | Store at 2 to 8°C for 6 months |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TRF2 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Rat |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Quantitative Sandwich |
| Samples | Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates |
| Detection Range | 0.05-20 ng/mL |
| Sensitivity | 0.022 ng/mL |
| Inter-assay CV | CV<10% |
| Intra-assay CV | CV<8% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 194,712 Da |
| NCBI Accession | ABA42953.1 |
| NCBI GeneID | 31773 |
| NCBI Official Full Name | TRF2 |
| NCBI Official Symbol | Trf2 |
| NCBI Official Synonym Full Names | TATA box binding protein-related factor 2 |
| NCBI Official Synonym Symbols | CG11195, CG18009, DmelCG18009, dTrf2, dTRF2, dTRF2L, dTRF2S, l(1)G0039, l(1)G0071, l(1)G0295, l(1)G0425, p79/TRF2, TLF, trf2, TRF2 |
| NCBI Protein Information | TATA box binding protein-related factor 2 |
| UniProt Gene Name | Trf2 |
| UniProt Protein Name | TATA box binding protein-related factor 2, isoform E |
| UniProt Synonym Gene Names | CG11195ImportedAutomatic assertion inferred from database entriesiEMBL:ABW09355.1, DmelCG18009ImportedAutomatic assertion inferred from database entriesiEMBL:ABW09355.1, dTRF2ImportedAutomatic assertion inferred from database entriesiEMBL:ABW09355.1, dTrf |
| UniProt Synonym Protein Names | TATA box binding protein-related factor 2, isoform E |
| Protein Family | Tricorn protease-interacting factor |
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