Rat Telomere Repeat Binding Factor 1 (TRF1) ELISA Kit

INQUIRY

CAT. NO.: TK130

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Product Overview	This sandwich Kit is designed for the precise quantitative detection of Rat Telomere Repeat Binding Factor 1 (also known as TRF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. This Kit utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) method. The plate has been pre-coated with Rat TRF1 antibody. TRF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TRF1 Antibody is added and binds to TRF1 in the sample. Following this, Streptavidin-HRP is introduced and binds to the Biotinylated TRF1 antibody. During a washing step, any unbound Streptavidin-HRP is removed. A substrate solution is then added, and color development occurs in proportion to the quantity of Rat TRF1. The reaction is halted by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Storage Conditions	Store at 2 to 8°C for 6 months
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TRF1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Rat
Shipping Conditions	Dry Ice

Product Details
Assay Type	Quantitative Sandwich
Samples	Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Detection Range	0.05-30 ng/mL
Sensitivity	0.026 ng/mL
Inter-assay CV	CV<10%
Intra-assay CV	CV<8%
Detection Wavelength	450 nm

Target Info
Molecular Weight	48,239 Da
NCBI Accession	NP_059523.2
NCBI GenBank Nucleotide	NM_017489.2
NCBI GeneID	7013
NCBI Official Full Name	telomeric repeat-binding factor 1 isoform 1
NCBI Official Symbol	TERF1
NCBI Official Synonym Full Names	telomeric repeat binding factor 1
NCBI Official Synonym Symbols	TRF, PIN2, TRF1, TRBF1, t-TRF1, hTRF1-AS
NCBI Protein Information	telomeric repeat-binding factor 1
UniProt Gene Name	TERF1
UniProt Protein Name	Telomeric repeat-binding factor 1
UniProt Synonym Gene Names	PIN2, TRBF1, TRF, TRF1
UniProt Synonym Protein Names	NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1
UniProt Primary Accession	P54274
UniProt Secondary Accession	Q15553, Q8NHT6, Q93029, A7XP29
Protein Family	Telomeric DNA-binding factor
NCBI Summary	This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
UniProt Comments	Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.