Rat Telomerase (TE) ELISA Kit

INQUIRY

CAT. NO.: TK061

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Application	Enzyme-linked immunosorbent assay for antigen detection.
Product Overview	This Kit employs the Double Antibody Sandwich ELISA technique. The plate comes pre-coated with an anti-Rat TE monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added to ELISA plate wells and subsequently washed with PBS or TBS following their respective additions. Avidin-peroxidase conjugates are then introduced to the wells. Following a thorough wash with PBS or TBS, TMB substrate is utilized for color development. TMB reacts with peroxidase activity, resulting in the formation of a blue product, which ultimately turns yellow upon the addition of the stop solution (Color Reagent C). The intensity of the color and the quantity of the target analyte in the sample exhibit a positive correlation.
Storage Conditions	Store all reagents at 2-8°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TE ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Rat
Shipping Conditions	Dry Ice

Product Details
Assay Type	Quantitative Sandwich
Samples	Rat Serum, Plasma, Cell Culture Supernatant
Detection Range	20-0.312 ng/mL
Sensitivity	Up to 0.06 ng/mL
Inter-assay CV	CV<= 12%
Intra-assay CV	CV<= 8%
Detection Wavelength	450 nm
Assay Time	1-4.5 h

Target Info
Molecular Weight	88,965 Da
NCBI Accession	NP_937983.2
NCBI GenBank Nucleotide	NM_198253.2
NCBI GeneID	7015
NCBI Official Full Name	telomerase reverse transcriptase isoform 1
NCBI Official Symbol	TERT
NCBI Official Synonym Full Names	telomerase reverse transcriptase
NCBI Official Synonym Symbols	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
NCBI Protein Information	telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2
OMIM	615134
UniProt Entry Name	TERT_HUMAN
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Gene Names	EST2, TCS1, TRT, TP2
UniProt Synonym Protein Names	HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
NCBI Summary	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
UniProt Comments	TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: Transferase; DNA-binding; Nucleolus; EC 2.7.7.49Chromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activityBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; telomere maintenance; positive regulation of Wnt receptor signaling pathway; RNA interference, production of siRNADisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1

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