Rat Telomerase Reverse Transcriptase (TERT) ELISA Kit

INQUIRY

CAT. NO.: TK065

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Product Overview	This TERT ELISA Kit is designed for the quantitative determination of Rat TERT and follows a solid-phase ELISA format. The TERT ELISA Kit employs a competitive enzyme immunoassay technique, utilizing a polyclonal anti-TERT antibody and a TERT-HRP conjugate. During the assay, the sample and buffer are incubated together with the TERT-HRP conjugate on a pre-coated plate for one hour. Following this incubation period, the wells are emptied and washed five times to remove any unbound substances. Next, the wells are incubated with a substrate for the HRP enzyme. The resulting enzyme-substrate reaction generates a blue-colored complex. To terminate the reaction, a stop solution is added, which subsequently changes the solution's color to yellow. The intensity of this yellow color is quantitatively measured spectrophotometrically at 450nm using a microplate reader. This color intensity is inversely proportional to the concentration of TERT, as TERT from the samples and TERT-HRP conjugate compete for binding sites on the anti-TERT antibody. Given the limited number of binding sites, as more sites are occupied by TERT from the sample, fewer sites are available to bind TERT-HRP conjugate. To determine the TERT concentration in each sample, a standard curve is constructed, correlating the intensity of the color (O.D.) to the concentration of standards. The TERT concentration in each sample can then be determined by interpolation from this standard curve.
Storage Conditions	Store all reagents at 2-8°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Rat
Shipping Conditions	Dry Ice

Product Details
Assay Type	Competitive
Samples	Serum, Plasma, Cell Culture Supernatants, Body Fluid, Tissue Homogenate
Sample Preparation	We recommend preliminary experimentation using undiluted samples or dilutions at ratios of 1:2 or 1:4. Please refrain from exceeding a 1:10 dilution, as it surpasses the prescribed dilution threshold for this kit.
Detection Range	100-2500 pg/mL
Sensitivity	1.0 pg/mL
Detection Wavelength	450 nm

Target Info
Molecular Weight	88,965 Da
NCBI Accession	O14746.1
NCBI GeneID	7015
NCBI Official Full Name	Telomerase reverse transcriptase
NCBI Official Symbol	TERT
NCBI Official Synonym Full Names	telomerase reverse transcriptase
NCBI Official Synonym Symbols	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
NCBI Protein Information	telomerase reverse transcriptase, telomerase catalytic subunit, telomerase-associated protein 2
OMIM	615134
UniProt Entry Name	TERT_HUMAN
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Gene Names	EST2, TCS1, TRT, TP2
UniProt Synonym Protein Names	HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Protein Family	Telomerase reverse transcriptase
NCBI Summary	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
UniProt Comments	TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: EC 2.7.7.49; DNA-binding; Transferase; NucleolusChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; RNA-directed RNA polymerase activity; protein homodimerization activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activityBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; telomere maintenance; positive regulation of Wnt receptor signaling pathway; RNA interference, production of siRNADisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1

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