Rat Telomerase Reverse Transcriptase (TERT) ELISA Kit

INQUIRY

CAT. NO.: TK064

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Application	Enzyme-linked immunosorbent assay for antigen detection.
Product Overview	This Kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technology. Initially, anti-TCF7L2 antibody is pre-coated onto 96-well plates. Subsequently, biotin-conjugated anti-TCF7L2 antibody is employed as the detection antibody. The process involves adding standards, test samples, and the biotin-conjugated detection antibody to the wells, followed by a wash with a wash buffer. Next, HRP-Streptavidin is introduced, and any unbound conjugates are thoroughly washed away using the wash buffer. To visualize the HRP enzymatic reaction, TMB substrates are utilized. TMB undergoes catalysis by HRP, resulting in the generation of a blue-colored product that transitions to yellow upon the addition of an acidic stop solution. The intensity of the yellow color is directly proportional to the amount of TCF7L2 captured from the sample within the plate. The optical density (O.D.) absorbance at 450nm is then measured using a microplate reader, allowing for the calculation of the concentration of TCF7L2.
Storage Conditions	Store at 4°C for 6 months
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Rat
Specificity	This assay has high sensitivity and excellent specificity for detection of TCF7L2. No significant cross-reactivity or interference between TCF7L2 and analogues was observed.
Shipping Conditions	Dry Ice

Product Details
Assay Type	Sandwich
Samples	Serum, Plasma, Tissue Homogenates, Other Biological Fluids
Detection Range	78-5000 pg/mL
Sensitivity	< 46.9 pg/mL
Inter-assay CV	CV<10%
Intra-assay CV	CV<8%
Detection Wavelength	450 nm

Target Info
NCBI Accession	NP_001079102.1
NCBI GenBank Nucleotide	NM_001085633.1
NCBI GeneID	373635
NCBI Official Full Name	telomerase reverse transcriptase
NCBI Official Symbol	tert.L
NCBI Official Synonym Full Names	telomerase reverse transcriptase L homeolog
NCBI Official Synonym Symbols	tp2, trt, est2, tcs1, tert, xTERT, tert-A, telomerase
NCBI Protein Information	telomerase reverse transcriptase
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Protein Family	Telomerase reverse transcriptase
UniProt Comments	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis ().

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