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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Phospho-Tuberin/TSC2 (Ser1254) Antibody

INQUIRY

CAT. NO.: AMA-0548

Target Information
UniProt ID	P49815

Product Details
Size	100 µL
Reactivity	Human, Mouse, Rat
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1254 of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB
Product Usage Information Dilution	Western Blotting (1:1000)
MW (Target)	200 kDa

Background
Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors Tuberin is directly phosphorylated at Thr1462 by Akt/PKB Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation.p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3.

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors Tuberin is directly phosphorylated at Thr1462 by Akt/PKB Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation.p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.