Phospho-(Ser/Thr) Akt Substrate Antibody

INQUIRY

CAT. NO.: AMA-0251

Product Details
Size	100 μL/300 µL
Reactivity	All Species
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with phospho-Akt substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB, IP, IHC
Product Usage Information Dilution	Western Blotting (1:1000); Immunoprecipitation (1:50); Immunohistochemistry (1:500)

Background
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr. Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation. While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3.

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr. Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation. While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3.

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