Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody

INQUIRY

CAT. NO.: AMA-0245

Product Details
Size	100 µL
Reactivity	All Species
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with Arg-X-Tyr/Phe-X-pSer-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB, IP
Product Usage Information Dilution	Western Blotting (1:1000); Immunoprecipitation (1:50)

Background
The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Binding of 14-3-3 is mediated through phospho-serine-containing proteins. Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXSXP and RXY/FXSXP (6). Motif 2 (RXY/FXSXP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR. Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS motif.

Background

The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Binding of 14-3-3 is mediated through phospho-serine-containing proteins. Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR. Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif.

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