Phospho-p73 (Tyr99) Antibody

INQUIRY

CAT. NO.: AMA-0319

Target Information
UniProt ID	O15350

Product Details
Size	100 µL
Reactivity	Human
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr99 of human p73. Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB
Product Usage Information Dilution	Western Blotting (1:1000)
MW (Target)	80 kDa

Background
The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 Another report, however, indicates that p300 does not acetylate full-length p73 in vivoWhile the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate.

Background

The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 Another report, however, indicates that p300 does not acetylate full-length p73 in vivoWhile the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.