Phospho-μ-Opioid Receptor (Ser375) Antibody

INQUIRY

CAT. NO.: AMA-0781

Target Information
UniProt ID	P35372

Product Details
Size	100 µL
Reactivity	Mouse
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human (homologous to Ser375 of mouse) μ-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB, IP, IF
Product Usage Information Dilution	Western Blotting (1:1000); Immunoprecipitation (1:100); Immunofluorescence (1:200)
MW (Target)	70-90 kDa

Background
The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization.

Background

The μ-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domains Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.