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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Phospho-LKB1 (Ser428) (C67A3) Rabbit Antibody

INQUIRY

CAT. NO.: AMA-0074

Target Information
UniProt ID	Q15831

Product Details
Size	100 μL
Reactivity	Human, Monkey, Mouse, Rat
Host	Rabbit
Source/Isotype	Rabbit
Storage	Store at -20°C.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser428 of human LKB1 (Ser431 of mouse).

Usage
Application	WB
Product Usage Information Dilution	Western Blotting (1:1000)
MW (Target)	54 kDa

Background
LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363.Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 as well as regulate apoptosis through PTEN signaling pathway suppression of Akt.

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363.Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 as well as regulate apoptosis through PTEN signaling pathway suppression of Akt.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.