Phospho-Gab2 (Ser159) Antibody

INQUIRY

CAT. NO.: AMA-0542

Target Information
UniProt ID	Q9UQC2

Product Details
Size	100 µL
Reactivity	Human, Mouse, Rat
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 of human Gab2. Antibodies are purified by protein A and peptide affinity chromatography.

Usage
Application	WB
Product Usage Information Dilution	Western Blotting (1:1000)
MW (Target)	98 kDa

Background
The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold Multiple tyrosine phosphorylation sites of Gab1 protein have been identified Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2Akt phosphorylates Gab2 at Ser159. This phosphorylation inhibits Gab2 tyrosine phosphorylation and downstream signal amplification.

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold Multiple tyrosine phosphorylation sites of Gab1 protein have been identified Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2Akt phosphorylates Gab2 at Ser159. This phosphorylation inhibits Gab2 tyrosine phosphorylation and downstream signal amplification.

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