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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Phospho-FoxO3a (Ser253) (D18H8) Rabbit Antibody

INQUIRY

CAT. NO.: AMA-0670

Target Information
UniProt ID	O43524

Product Details
Size	100 µL
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Source/Isotype	Rabbit IgG
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FoxO3a protein.

Usage
Application	WB
Product Usage Information Dilution	Western Blotting (1:1000)
MW (Target)	97 kDa

Background
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1)FoxO3a is phosphorylated by Akt at several sites, including Thr32, Ser253 and Ser315. FoxO3a associates with 14-3-3 proteins following Akt phosphorylation and is retained in the cytoplasm. In the absence of survival factors, FoxO3a is dephosphorylated, translocates to the nucleus, and triggers cell death by a Fas ligand-dependent mechanism.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1)FoxO3a is phosphorylated by Akt at several sites, including Thr32, Ser253 and Ser315. FoxO3a associates with 14-3-3 proteins following Akt phosphorylation and is retained in the cytoplasm. In the absence of survival factors, FoxO3a is dephosphorylated, translocates to the nucleus, and triggers cell death by a Fas ligand-dependent mechanism.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.