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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Phospho-Bim (Ser77) (D4H12) Rabbit Antibody

INQUIRY

CAT. NO.: AMA-0449

Target Information
UniProt ID	O43521

Product Details
Size	100 µL
Reactivity	Human, Mouse
Host	Rabbit
Source/Isotype	Rabbit IgG
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser77 of human Bim protein.

Usage
Application	WB, IP
Product Usage Information Dilution	Western Blotting (1:1000); Immunoprecipitation (1:100)
MW (Target)	26 kDa

Background
Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1 Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis Apoptotic activity of these longer isoforms may be regulated by phosphorylation Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.MAP kinase dependent phosphorylation of Bim at multiple sites, including Ser55, Ser65, and Ser73 in mouse (Ser59, Ser69, and Ser77 in human), can promote proteasomal degradation of Bim and inhibition of apoptosis.

Background

Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1 Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis Apoptotic activity of these longer isoforms may be regulated by phosphorylation Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.MAP kinase dependent phosphorylation of Bim at multiple sites, including Ser55, Ser65, and Ser73 in mouse (Ser59, Ser69, and Ser77 in human), can promote proteasomal degradation of Bim and inhibition of apoptosis.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.