CAT. NO.: PA003
| General Info | |
|---|---|
| Size | 96-well plate/384-well plate |
| Product Overview | The Rat Aging RT2 Profiler PCR Array profiles the expression of 84 genes altered during aging, a major biological process and a risk factor for many diseases. To gain new insights into the process of aging and identify potentially important genes and biomarkers, many microarray studies have been conducted in several species, including humans, either by comparing various young and old tissues or by comparing samples across the organism's lifespan. Characterization of the associated biological processes has found that age-related gene expression changes involve alterations in the expression of genes related to apoptosis, cell cycle, cell senescence, and inflammation, as well as mitochondrial genes. The other common denominators of aging include altered expression of genes linked to epigenetic alterations, genomic instability, telomere attrition, and proteostasis - the stabilization of correctly folded proteins. These molecular markers and processes reflect a combination of degeneration and transcriptional responses to aging. Analyzing the expression of these genes can provide a better understanding of how transcriptional changes relate to the aging process. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to aging with this array. |
| Storage Conditions | Store at -20°C |
| Notes | Before ordering, please choose the right type of array according to the model of your qPCR instrument, and after receiving the product, please confirm whether the received array matches with your qPCR instrument in time. |
| Reactivity | Rat |
| Shipping Conditions | Transport at 4°C |
| Product Details | |
|---|---|
| Quality Control | The gene primers detected in the gene qPCR array, analyzed by the experimental verification results, the melting curves were all single sharp edges, and the electrophoretic bands were all single bands, which indicated that these primers were specific amplification primers, and the verification was qualified. PTC (positive control) reaction wells, analyzed from the melting curve, all showed a single sharp edge, and analyzed from the amplification curve, the CT value was between 18 and 22. RTC (spike-in reverse transcription control) reaction wells, analyzed from the melting curve are single sharp edges, analyzed from the amplification curve, the CT value is between 17 and 23. The CT value for HGDC should be greater than 35, indicating that there is no genomic contamination in the sample. The CT values of three replicates of RTC and PTC should be close to each other, indicating good reproducibility of the experiment. Plate-to-plate (within batch) repeatability of 0.99 or more is highly reproducible. |
| Highlights | High sensitivity and low sample usage. Wide linear range allows the simultaneous detection of genes with widely varying expression levels. Detection primers for each gene are optimized to ensure high amplification rate and single product. Highly reproducible, with an average difference in Ct values of only 0.25 cycles. Wide coverage, catalog products include pathway analysis and disease research. Customized products can be selected and set up according to customer requirements. Convenient data analysis, dedicated software facilitates data statistics and analysis. |
| Target Info | |
|---|---|
| Gene Primer List | Gene1 Angel2 NM_001135119 Gene2 Anxa3 NM_012823 Gene3 Anxa5 NM_013132 Gene4 Arid1a NM_001106635 Gene5 Arl6ip6 NM_001024310 Gene6 Bub1b XM_342494 Gene7 C1qa NM_001008515 Gene8 C1qb NM_019262 Gene9 C1qc NM_001008524 Gene10 C1s NM_138900 Gene11 C3 NM_016994 Gene12 C3ar1 NM_032060 Gene13 C4a NM_031504 Gene14 C4b NM_001002805 Gene15 C5ar1 NM_053619 Gene16 Calb1 NM_031984 Gene17 Casp1 NM_012762 Gene18 Ccr1 NM_020542 Gene19 Cd14 NM_021744 Gene20 Cd163 NM_001107887 Gene21 Cdkn1c NM_182735 Gene22 Cfh NM_130409 Gene23 Cfhr1 NM_001044227 Gene24 Clu NM_053021 Gene25 Col1a1 NM_053304 Gene26 Cx3cl1 NM_134455 Gene27 Cxcl16 NM_001017478 Gene28 Elavl1 NM_001108848 Gene29 Eml1 NM_001025741 Gene30 Ep300 XM_001076610 Gene31 Fbxl16 NM_001009504 Gene32 Fcer1g NM_001131001 Gene33 Fcgbp NM_001164657 Gene34 Fcgr1a NM_001100836 Gene35 Fcgr2a NM_053843 Gene36 Fcgr2b NM_175756 Gene37 Foxo1 NM_001191846 Gene38 Gfap NM_017009 Gene39 Hsf1 NM_024393 Gene40 Jakmip3 NM_001163277 Gene41 Lmna NM_001002016 Gene42 Lmnb1 NM_053905 Gene43 Lmnb2 XM_216850 Gene44 LOC100362548 XM_002728290 Gene45 Lsm5 NM_001107289 Gene46 Ltf NM_001106864 Gene47 Lyzl1 NM_001108882 Gene48 Mbp NM_017026 Gene49 Mrpl43 NM_001107598 Gene50 Ndufb11 NM_001106756 Gene51 Panx1 NM_199397 Gene52 Pdcd6 NM_001107452 Gene53 Phf3 NM_001108791 Gene54 Polrmt NM_001106766 Gene55 Pot1 NM_001024322 Gene56 Rap1a NM_001005765 Gene57 RGD1564788 XM_006222255 Gene58 Rnf144b NM_001108881 Gene59 S100a8 NM_053822 Gene60 S100a9 NM_053587 Gene61 Scn2b NM_012877 Gene62 Sirt3 NM_001106313 Gene63 Sirt6 NM_001031649 Gene64 Smad2 NM_019191 Gene65 Snap23 NM_022689 Gene66 Terf1 NM_001012464 Gene67 Terf2 NM_001108448 Gene68 Tfam NM_031326 Gene69 Tfb1m NM_181474 Gene70 Tfb2m NM_001008293 Gene71 Tinf2 NM_001006962 Gene72 Tlr2 NM_198769 Gene73 Tlr4 NM_019178 Gene74 Tmem135 NM_001013896 Gene75 Tmem33 NM_021671 Gene76 Tollip NM_001109668 Gene77 Tpp1 NM_031357 Gene78 Txnip NM_001008767 Gene79 Vps13c XR_348616 Gene80 Vwa5a NM_198755 Gene81 Zbtb10 NM_024489 Gene82 Zfp9 NM_001127635 Gene83 Zfr NM_001270972 Gene84 Zmpste24 NM_001107974 |
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CD BioScience is a research service provider focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
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