CAT. NO.: PA008
| General Info | |
|---|---|
| Size | 250-well plate |
| Product Overview | The Mouse Aging RT2 Profiler PCR Array profiles the expression of 84 genes altered during aging, a major biological process and a risk factor for many diseases. To gain new insights into the process of aging and identify potentially important genes and biomarkers, many microarray studies have been conducted in several species, including humans, either by comparing various young and old tissues or by comparing samples across the organism's lifespan. Characterization of the associated biological processes has found that age-related gene expression changes involve alterations in the expression of genes related to apoptosis, cell cycle, cell senescence, and inflammation, as well as mitochondrial genes. The other common denominators of aging include altered expression of genes linked to epigenetic alterations, genomic instability, telomere attrition, and proteostasis - the stabilization of correctly folded proteins. These molecular markers and processes reflect a combination of degeneration and transcriptional responses to aging. Analyzing the expression of these genes can provide a better understanding of how transcriptional changes relate to the aging process. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to aging with this array. |
| Storage Conditions | Store at -20°C |
| Notes | Before ordering, please choose the right type of array according to the model of your qPCR instrument, and after receiving the product, please confirm whether the received array matches with your qPCR instrument in time. |
| Reactivity | Mouse |
| Shipping Conditions | Transport at 4°C |
| Product Details | |
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| Quality Control | The gene primers detected in the gene qPCR array, analyzed by the experimental verification results, the melting curves were all single sharp edges, and the electrophoretic bands were all single bands, which indicated that these primers were specific amplification primers, and the verification was qualified. PTC (positive control) reaction wells, analyzed from the melting curve, all showed a single sharp edge, and analyzed from the amplification curve, the CT value was between 18 and 22. RTC (spike-in reverse transcription control) reaction wells, analyzed from the melting curve are single sharp edges, analyzed from the amplification curve, the CT value is between 17 and 23. The CT value for HGDC should be greater than 35, indicating that there is no genomic contamination in the sample. The CT values of three replicates of RTC and PTC should be close to each other, indicating good reproducibility of the experiment. Plate-to-plate (within batch) repeatability of 0.99 or more is highly reproducible. |
| Highlights | High sensitivity and low sample usage. Wide linear range allows the simultaneous detection of genes with widely varying expression levels. Detection primers for each gene are optimized to ensure high amplification rate and single product. Highly reproducible, with an average difference in Ct values of only 0.25 cycles. Wide coverage, catalog products include pathway analysis and disease research. Customized products can be selected and set up according to customer requirements. Convenient data analysis, dedicated software facilitates data statistics and analysis. |
| Target Info | |
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| Gene Primer List | Gene1 Angel2 NM_021421 Gene2 Anxa3 NM_013470 Gene3 Anxa5 NM_009673 Gene4 Arid1a NM_001080819 Gene5 Arl6ip6 NM_022989 Gene6 Bub1b NM_009773 Gene7 C1qa NM_007572 Gene8 C1qb NM_009777 Gene9 C1qc NM_007574 Gene10 C1s NM_144938 Gene11 C3 NM_009778 Gene12 C3ar1 NM_009779 Gene13 C4a NM_011413 Gene14 C4b NM_009780 Gene15 C5ar1 NM_007577 Gene16 Calb1 NM_009788 Gene17 Casp1 NM_009807 Gene18 Ccr1 NM_009912 Gene19 Cd14 NM_009841 Gene20 Cd163 NM_053094 Gene21 Cdkn1c NM_009876 Gene22 Cfh NM_009888 Gene23 Cfhr1 NM_015780 Gene24 Clu NM_013492 Gene25 Cx3cl1 NM_009142 Gene26 Cxcl16 NM_023158 Gene27 Elavl1 NM_010485 Gene28 Elp3 NM_028811 Gene29 Eml1 NM_001043335 Gene30 Ep300 NM_177821 Gene31 Fbxl16 NM_001164225 Gene32 Fcer1g NM_010185 Gene33 Fcgbp NM_001122603 Gene34 Fcgr1 NM_010186 Gene35 Fcgr2b NM_010187 Gene36 Fcgr3 NM_010188 Gene37 Foxo1 NM_019739 Gene38 Gfap NM_010277 Gene39 Gsta1 NM_008181 Gene40 Hsf1 NM_008296 Gene41 Jakmip3 NM_028708 Gene42 Lmna NM_019390 Gene43 Lmnb1 NM_010721 Gene44 Lmnb2 NM_010722 Gene45 Lsm5 NM_025520 Gene46 Ltf NM_008522 Gene47 Mbp NM_010777 Gene48 Mrpl43 NM_053164 Gene49 Ndufb11 NM_019435 Gene50 Panx1 NM_019482 Gene51 Pdcd6 NM_011051 Gene52 Phf3 NM_001081080 Gene53 Polrmt NM_172551 Gene54 Pot1a NM_133931 Gene55 Rap1a NM_145541 Gene56 Rnf144b NM_146042 Gene57 S100a8 NM_013650 Gene58 S100a9 NM_009114 Gene59 Scn2b NM_001014761 Gene60 Sirt1 NM_019812 Gene61 Sirt3 NM_022433 Gene62 Sirt6 NM_181586 Gene63 Smad2 NM_010754 Gene64 Snap23 NM_009222 Gene65 Terf1 NM_009352 Gene66 Terf2 NM_009353 Gene67 Tfam NM_009360 Gene68 Tfb1m NM_146074 Gene69 Tfb2m NM_008249 Gene70 Tinf2 NM_145705 Gene71 Tlr2 NM_011905 Gene72 Tlr4 NM_021297 Gene73 Tmem135 NM_028343 Gene74 Tmem33 NM_028975 Gene75 Tollip NM_023764 Gene76 Tpp1 NM_009906 Gene77 Txnip NM_023719 Gene78 Vps13c NM_177184 Gene79 Vwa5a NM_172767 Gene80 Wrn NM_011721 Gene81 Zbtb10 NM_177660 Gene82 Zfp9 NM_011763 Gene83 Zfr NM_011767 Gene84 Zmpste24 NM_172700 |
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CD BioScience is a research service provider focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
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