CAT. NO.: PA009
| General Info | |
|---|---|
| Size | 96-well plate/384-well plate |
| Product Overview | The Human Aging RT2 Profiler PCR Array profiles the expression of 84 genes altered during aging, a major biological process and a risk factor for many diseases. To gain new insights into the process of aging and identify potentially important genes and biomarkers, many microarray studies have been conducted in several species, including humans, either by comparing various young and old tissues or by comparing samples across the organism's lifespan. Characterization of the associated biological processes has found that age-related gene expression changes involve alterations in the expression of genes related to apoptosis, cell cycle, cell senescence, and inflammation, as well as mitochondrial genes. The other common denominators of aging include altered expression of genes linked to epigenetic alterations, genomic instability, telomere attrition, and proteostasis - the stabilization of correctly folded proteins. These molecular markers and processes reflect a combination of degeneration and transcriptional responses to aging. Analyzing the expression of these genes can provide a better understanding of how transcriptional changes relate to the aging process. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to aging with this array. |
| Storage Conditions | Store at -20°C |
| Notes | Before ordering, please choose the right type of array according to the model of your qPCR instrument, and after receiving the product, please confirm whether the received array matches with your qPCR instrument in time. |
| Reactivity | Human |
| Shipping Conditions | Transport at 4°C |
| Product Details | |
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| Quality Control | The gene primers detected in the gene qPCR array, analyzed by the experimental verification results, the melting curves were all single sharp edges, and the electrophoretic bands were all single bands, which indicated that these primers were specific amplification primers, and the verification was qualified. PTC (positive control) reaction wells, analyzed from the melting curve, all showed a single sharp edge, and analyzed from the amplification curve, the CT value was between 18 and 22. RTC (spike-in reverse transcription control) reaction wells, analyzed from the melting curve are single sharp edges, analyzed from the amplification curve, the CT value is between 17 and 23. The CT value for HGDC should be greater than 35, indicating that there is no genomic contamination in the sample. The CT values of three replicates of RTC and PTC should be close to each other, indicating good reproducibility of the experiment. Plate-to-plate (within batch) repeatability of 0.99 or more is highly reproducible. |
| Highlights | High sensitivity and low sample usage. Wide linear range allows the simultaneous detection of genes with widely varying expression levels. Detection primers for each gene are optimized to ensure high amplification rate and single product. Highly reproducible, with an average difference in Ct values of only 0.25 cycles. Wide coverage, catalog products include pathway analysis and disease research. Customized products can be selected and set up according to customer requirements. Convenient data analysis, dedicated software facilitates data statistics and analysis. |
| Target Info | |
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| Gene Primer List | Gene1 BUB1B Gene2 MRPL43 Gene3 POLRMT Gene4 TFAM Gene5 TFB1M Gene6 TFB2M Gene7 ZMPSTE24 Gene8 POT1 Gene9 RAP1A Gene10 TERF1 Gene11 TERF2 Gene12 TINF2 Gene13 TPP1 Gene14 NDUFB11 Gene15 SIRT1 Gene16 SIRT3 Gene17 SIRT6 Gene18 ARL6IP6 Gene19 FOXO1 Gene20 HSF1 Gene21 JAKMIP3 Gene22 RNF144B Gene23 TXNIP Gene24 VPS13C Gene25 LMNA Gene26 LMNB1 Gene27 LMNB2 Gene28 CALB1 Gene29 GFAP Gene30 MBP Gene31 SCN2B Gene32 SNAP23 Gene33 ARID1A Gene34 ELP3 Gene35 EP300 Gene36 FBXL16 Gene37 ZBTB10 Gene38 ZFR Gene39 ZNF25 Gene40 ELAVL1 Gene41 LSM5 Gene42 ANGEL2 Gene43 ANXA3 Gene44 ANXA5 Gene45 C1QA Gene46 C1QB Gene47 C1QC Gene48 C1S Gene49 C3 Gene50 C3AR1 Gene51 C5AR1 Gene52 CCR1 Gene53 CD14 Gene54 CD163 Gene55 CFH Gene56 CX3CL1 Gene57 CXCL16 Gene58 FCER1G Gene59 FCGBP Gene60 FCGR1A Gene61 FCGR2A Gene62 FCGR3B Gene63 LTF Gene64 LYZ Gene65 PANX1 Gene66 S100A8 Gene67 S100A9 Gene68 TMEM135 Gene69 TMEM33 Gene70 TLR2 Gene71 TLR4 Gene72 TOLLIP Gene73 CASP1 Gene74 CLU Gene75 PDCD6 Gene76 CDKN1C Gene77 VWA5A Gene78 WRN Gene79 COL1A1 Gene80 COL3A1 Gene81 EML1 Gene82 GSTA Gene83 PHF3 Gene84 SMAD2 |
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CD BioScience is a research service provider focused on aging research, specializing in providing research on aging mechanisms, preclinical drug discovery and development, and the development of healthcare and skincare products.
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