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Project Description

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

p21 Waf1/Cip1 (12D1) Rabbit Antibody (BSA and Azide Free)

INQUIRY

CAT. NO.: AMA-0421

Target Information
UniProt ID	P38936

Product Details
Size	100 µg
Reactivity	Human, Monkey
Host	Rabbit
Source/Isotype	Rabbit IgG
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p21.

Usage
Application	WB, IHC, IF, F
MW (Target)	21 kDa

Background
The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.