p14 ARF (4C6/4) Mouse Antibody

INQUIRY

CAT. NO.: AMA-0288

Target Information
UniProt ID	Q8N726

Product Details
Size	100 µL
Reactivity	Human
Host	Mouse
Source/Isotype	Mouse IgG2a
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a recombinant His-tagged p14 ARF protein.

Usage
Application	WB, IHC
Product Usage Information Dilution	Western Blotting (1:1000); Immunohistochemistry (1:50)
MW (Target)	14 kDa

Background
Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors. Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression. Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis. While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis.

Background

Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors. Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression. Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis. While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.