Oncogene-Induced Cellular Senescence Model Customization Services

Oncogene-induced senescence (OIS) manifests as a stable and strong antiproliferative reaction to oncogenic signaling that occurs because of either an activating oncogene mutation or the loss of tumor-suppressor gene function. The modeling of oncogene-induced cellular senescence helps researchers identify potential therapeutic targets to control senescence responses in cancer cells.

Fig. 1 OIS in complex biological systems. (Galluzzi L, Vitale I, 2018)

CD BioSciences is dedicated to providing cutting-edge services in the field of aging research. Our expertise lies in offering customized solutions for constructing oncogene-induced cellular senescence models.

Oncogene-Induced Cellular Senescence Model Customization Options

Oncogene selection

Our team of experts works with clients to explore the extensive range of oncogenes to identify those that meet their scientific research goals. The oncogenes H-RAS, K-RAS, and BRAF frequently serve as inducers of senescence across multiple cell types.

Cell line selection

Different cell lines exhibit varying susceptibilities to OIS and selecting a cell line that closely mimics the biological context of your study is essential. We assist our clients in evaluating the available options and choosing a cell line that best aligns with the research goals. Whether human fibroblasts, epithelial cells, or immortalized cell lines, we have the expertise to guide clients in making an informed decision.

Gene delivery methods

With our diverse gene delivery methods, we can customize the approach to suit the client's experimental needs and maximize the success of your oncogene-induced cellular senescence models.

Workflow of Customizing Oncogene-Induced Cellular Senescence Models

• Experimental design and execution. Through collaboration with our clients, our expert team develops experimental designs that meet specific research objectives. Our services include supporting clients with the determination of the time points for oncogene induction, appropriate dosing levels, and monitoring parameters. Furthermore, we help clients establish appropriate control groups to ensure the validity of the client's experimental results. Leveraging our extensive experience in experimental design and execution, we can help clients construct oncogene-induced cellular senescence models.
• Phenotypic characterization. We provide comprehensive support in phenotypic characterization, enabling clients to delve into the cellular changes associated with senescence. We offer a wide range of assays and techniques to evaluate the effects of OIS, such as senescence-associated β-galactosidase (SA-β-gal) staining, senescence-associated secretory phenotype (SASP) analysis, telomere length analysis, cell proliferation assays, cell cycle analysis.
• Data analysis and interpretation. Our team provides support to clients for data analysis while identifying statistically significant results between experimental and control groups.

CD BioSciences offers oncogene-induced cellular senescence model customization services, providing tailored solutions for clients in the field of aging biology. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Galluzzi L, Vitale I. Oncogene-induced senescence and tumor control in complex biological systems. Cell Death Differ, 2018, 25 (6): 1005-1006.