CAT. NO.: TK048
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This TERT ELISA Kit is designed for the quantitative determination of Mouse TERT and employs a solid-phase ELISA format. The TERT ELISA Kit utilizes the competitive enzyme immunoassay technique, utilizing a polyclonal anti-TERT antibody and a TERT-HRP conjugate. During the assay, the sample and buffer are incubated along with the TERT-HRP conjugate on a pre-coated plate for one hour. Following this incubation period, the wells are emptied and washed five times. Subsequently, the wells are subjected to incubation with a substrate for the HRP enzyme. The product of the enzyme-substrate reaction forms a blue-colored complex. To halt the reaction, a stop solution is introduced, turning the solution yellow. The intensity of the yellow color is measured spectrophotometrically at 450nm using a microplate reader. This intensity of color is inversely proportional to the concentration of TERT because TERT from the samples and TERT-HRP conjugate compete for binding sites on the anti-TERT antibody. Given that the number of available binding sites is limited, as more sites are occupied by TERT from the sample, fewer sites are available to bind TERT-HRP conjugate. To determine the TERT concentration in each sample, a standard curve is generated that correlates the intensity of the color (O.D.) to the concentration of standards. The TERT concentration in each sample can then be determined by interpolating it from this standard curve. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Mouse |
| Specificity | This assay recognizes recombinant and natural mouse Telomerase reverse transcriptase. No significant cross-reactivity or interference was observed. |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Competitive |
| Samples | Serum, Plasma, Cell Culture Supernatants, Body Fluid, Tissue Homogenate |
| Sample Preparation | We recommend preliminary experimentation using undiluted samples or dilutions at ratios of 1:2 or 1:4. Please refrain from exceeding a 1:10 dilution, as it surpasses the prescribed dilution threshold for this kit. |
| Sensitivity | 0.1 ng/mL |
| Inter-assay CV | CV<=7.7% |
| Intra-assay CV | CV<=6.3% |
| Detection Wavelength | 450 nm |
| Assay Time | 3-5 h |
| Target Info | |
|---|---|
| Molecular Weight | 127,978 Da |
| UniProt Primary Accession | O70372 |
| Protein Family | Telomerase reverse transcriptase |
| NCBI Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Comments | TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.Protein type: DNA-binding; EC 2.7.7.49; Transferase; NucleolusChromosomal Location of Human Ortholog: 5p15.33Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolusMolecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activityBiological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; positive regulation of Wnt receptor signaling pathway; RNA interference, production of siRNA; telomere maintenanceDisease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1 |
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