Mouse Telomerase Reverse Transcriptase (TERT) ELISA Kit

INQUIRY

CAT. NO.: TK047

General Info
Size	48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells
Product Overview	This sandwich Kit is designed for the precise quantitative detection of Mouse Telomerase Reverse Transcriptase (also known as TERT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. This Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse TERT antibody. TERT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TERT Antibody is added and binds to TERT in the sample. Following this, Streptavidin-HRP is introduced and binds to the Biotinylated TERT antibody. During a washing step, any unbound Streptavidin-HRP is removed. A substrate solution is then added, and color development occurs in proportion to the quantity of Mouse TERT. The reaction is halted by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Storage Conditions	Store at 2 to 8°C for 6 months
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Mouse
Shipping Conditions	Dry Ice

Product Details
Assay Type	Quantitative Sandwich
Samples	Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Detection Range	20-3500 ng/L
Sensitivity	9.46 ng/L
Inter-assay CV	CV<10%
Intra-assay CV	CV<8%
Detection Wavelength	450 nm

Target Info
Molecular Weight	90,226 Da
NCBI Accession	BAC11010.1
NCBI GeneID	7015
NCBI Official Full Name	telomerase reverse transcriptase
NCBI Official Symbol	TERT
NCBI Official Synonym Full Names	telomerase reverse transcriptase
NCBI Official Synonym Symbols	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
NCBI Protein Information	telomerase reverse transcriptase
UniProt Gene Name	TERT
UniProt Protein Name	Telomerase reverse transcriptase
UniProt Synonym Gene Names	EST2, TCS1, TRT, TP2
UniProt Synonym Protein Names	HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2
UniProt Primary Accession	O14746
UniProt Secondary Accession	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Protein Family	Telomerase reverse transcriptase
NCBI Summary	Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
UniProt Comments	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

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