CAT. NO.: TK047
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This sandwich Kit is designed for the precise quantitative detection of Mouse Telomerase Reverse Transcriptase (also known as TERT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. This Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse TERT antibody. TERT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TERT Antibody is added and binds to TERT in the sample. Following this, Streptavidin-HRP is introduced and binds to the Biotinylated TERT antibody. During a washing step, any unbound Streptavidin-HRP is removed. A substrate solution is then added, and color development occurs in proportion to the quantity of Mouse TERT. The reaction is halted by adding an acidic stop solution, and the absorbance is measured at 450 nm. |
| Storage Conditions | Store at 2 to 8°C for 6 months |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Mouse |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Quantitative Sandwich |
| Samples | Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates |
| Detection Range | 20-3500 ng/L |
| Sensitivity | 9.46 ng/L |
| Inter-assay CV | CV<10% |
| Intra-assay CV | CV<8% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 90,226 Da |
| NCBI Accession | BAC11010.1 |
| NCBI GeneID | 7015 |
| NCBI Official Full Name | telomerase reverse transcriptase |
| NCBI Official Symbol | TERT |
| NCBI Official Synonym Full Names | telomerase reverse transcriptase |
| NCBI Official Synonym Symbols | TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1 |
| NCBI Protein Information | telomerase reverse transcriptase |
| UniProt Gene Name | TERT |
| UniProt Protein Name | Telomerase reverse transcriptase |
| UniProt Synonym Gene Names | EST2, TCS1, TRT, TP2 |
| UniProt Synonym Protein Names | HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2 |
| UniProt Primary Accession | O14746 |
| UniProt Secondary Accession | O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46 |
| Protein Family | Telomerase reverse transcriptase |
| NCBI Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Comments | Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis. |
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