CAT. NO.: TK114
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This quantitative sandwich ELISA Kit is for lab reagent/research use only! This Kit is intended to be used to determine the level of POD in undiluted body fluids and tissue homogenates. This Kit is not suitable for assaying non-biological sources of substances. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the POT1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Mouse |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Quantitative Sandwich |
| Samples | Body Fluids, Tissue Homogenates |
| Detection Range | 31.2-1000 U/L |
| Sensitivity | 5.0 U/L |
| Inter-assay CV | CV<15% |
| Intra-assay CV | CV<15% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 71,442 Da |
| NCBI Accession | NP_056265.2 |
| NCBI GenBank Nucleotide | NM_015450.2 |
| NCBI GeneID | 25913 |
| NCBI Official Full Name | protection of telomeres protein 1 isoform 1 |
| NCBI Official Symbol | POT1 |
| NCBI Official Synonym Full Names | protection of telomeres 1 |
| NCBI Official Synonym Symbols | CMM10, HPOT1 |
| NCBI Protein Information | protection of telomeres protein 1, protection of telomeres 1 homolog, POT1-like telomere end-binding protein |
| OMIM | 606478 |
| UniProt Entry Name | POTE1_HUMAN |
| UniProt Gene Name | POT1 |
| UniProt Protein Name | Protection of telomeres protein 1 |
| UniProt Synonym Gene Names | hPot1 |
| UniProt Synonym Protein Names | POT1-like telomere end-binding protein |
| UniProt Primary Accession | Q9NUX5 |
| UniProt Secondary Accession | O95018, Q5MJ36, Q9H662, Q9NW19, Q9UG95 |
| Protein Family | Potassium transporter |
| NCBI Summary | This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| UniProt Comments | POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 7q31.33Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleusMolecular protein binding; DEAD/H-box RNA helicase binding; single-stranded telomeric DNA bindingBiological Process: positive regulation of helicase activity; telomere assembly; negative regulation of telomerase activity; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; telomere maintenance; negative regulation of telomere maintenance via telomerase; DNA duplex unwinding; positive regulation of telomere maintenance via telomeraseDisease: Melanoma, Cutaneous Malignant, Susceptibility To, 10 |
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