Mitochondrial Genome Sequencing for Aging Studies

Mitochondrial genome sequencing involves the identification and analysis of the complete set of genetic material present in the mitochondria. Heteroplasmic mutations in the mitochondrial genomes accumulate with age. Modern sequencing techniques allow the quantitation and localization of mutations and deletions. CD BioSciences harnesses state-of-the-art technologies, including next-generation sequencing platforms and cutting-edge bioinformatics tools, to deliver high-quality mitochondrial sequencing results.

Overview of Our Mitochondrial Genome Sequencing Services

At CD BioSciences, our approach used for mitochondrial sequencing is to amplify, by long-range PCR, the mitochondrial genome to enrich for the mtDNA and focus sequencing. By sequencing the mitochondrial genome at high depth, mutations occurring at a rate as low as 0.1 to 1% can be quantified.

After sequencing, we compare the obtained sequences with a reference mitochondrial genome sequence to identify genetic variants, including point mutations and deletions. Then, we apply filtering criteria to focus on potential variants of aging and aging-related diseases.

Guidelines for Sample Preparation

CD BioSciences understands the importance of sample setup and collection in the study of investigating aging with mitochondrial genome sequencing. We provide comprehensive guidelines to ensure optimal results.

• Cohort selection
  Choose a well-defined cohort of study participants that represents the target population you want to study. Consider factors such as age range, gender balance, and any relevant health conditions that may affect the mitochondrial genome.
• Sample collection
  Follow standardized sample collection protocols to minimize variability and follow best practices. This typically includes the collection of tissue, blood, saliva, or other appropriate biological samples. Below are recommendations on the quantities of different biological samples for sequencing.

Our Workflow of Mitochondrial Genome Sequencing

• Sample collection and isolation
  We provide detailed guidelines for sample collection to ensure the best possible quality of mitochondrial DNA. By employing advanced isolation techniques, we extract highly purified mitochondrial DNA, minimizing contamination and preserving sample integrity.
• Library preparation
  Our experienced scientists employ cutting-edge library preparation methods, customized for mitochondrial DNA sequencing. This step involves fragmenting the DNA and constructing libraries suitable for next-generation sequencing platforms.
• Next-generation sequencing
  We leverage advanced next-generation sequencing technologies, such as Illumina sequencing platforms, to obtain high-quality sequence data. This step enables comprehensive coverage of the mitochondrial genome, yielding detailed insights into the genetic composition.
• Bioinformatics analysis
  Our bioinformatics analysis pipeline focuses on accurately mapping the obtained sequences to the reference mitochondrial genome. This allows us to identify genetic variants, structural rearrangements, and potential disease-associated mutations.

Bioinformatics Analysis of Sequencing Data for Aging Studies

Our highly trained bioinformaticians employ cutting-edge algorithms and tools to deliver comprehensive results for aging studies, including genome assembly, gene annotation, codon analysis, circle mapping, SNP/Indel, Ka/Ks analysis, evolutionary tree construction, covariance analysis, mVISTA, IR expansion-contraction, homologous gene analysis, comparative circle mapping, and other customized analyses.

CD BioSciences, with its cutting-edge technologies, expert team, and customized solutions, empowers our clients to delve deep into the secrets of mitochondrial genetics in aging. If you are interested in our services, please feel free to contact us or make an online inquiry.