CAT. NO.: TK107
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This sandwich Kit is designed for the precise quantitative detection of human Telomere Repeat Binding Factor 1 (also known as TRF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. This Kit utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) method. The plate has been pre-coated with human TRF1 antibody. TRF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human TRF1 Antibody is added and binds to TRF1 in the sample. Following this, Streptavidin-HRP is introduced and binds to the Biotinylated TRF1 antibody. During a washing step, any unbound Streptavidin-HRP is removed. A substrate solution is then added, and color development occurs in proportion to the quantity of human TRF1. The reaction is halted by adding an acidic stop solution, and the absorbance is measured at 450 nm. |
| Storage Conditions | Store at 2 to 8°C for 6 months |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TRF1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Human |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Quantitative Sandwich |
| Samples | Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates |
| Detection Range | 0.05-30 ng/mL |
| Sensitivity | 0.027 ng/mL |
| Inter-assay CV | CV<10% |
| Intra-assay CV | CV<8% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 33,017 Da |
| NCBI Accession | NP_849789.1 |
| NCBI GenBank Nucleotide | NM_179458.3 |
| NCBI GeneID | 841418 |
| NCBI Official Full Name | telomere repeat binding factor 1 |
| NCBI Official Symbol | TRB1 |
| NCBI Official Synonym Symbols | ATTRB1, F2J10.16, F2J10_16, telomere repeat binding factor 1, TELOMERE REPEAT BINDING FACTOR 1 |
| NCBI Protein Information | telomere repeat binding factor 1 |
| UniProt Gene Name | TRB1 |
| UniProt Protein Name | Telomere repeat-binding factor 1 |
| UniProt Synonym Gene Names | AtTRB1 |
| UniProt Synonym Protein Names | MYB transcription factor |
| UniProt Primary Accession | Q8VWK4 |
| UniProt Secondary Accession | Q8LA70, Q8VX39, Q9LPL9 |
| Protein Family | Telomeric DNA-binding factor |
| NCBI Summary | Encodes a telomeric DNA binding protein. In vitro, an N-terminal Myb domain enables it to interact with double-stranded telomeric repeats. |
| UniProt Comments | Binds preferentially double-stranded telomeric repeats. |
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