Human Telomere Length Quantification qPCR Assay Kit

INQUIRY

CAT. NO.: TK003

General Info
Size	100 tests/500 tests
Product Overview	Accurate and consistent quantification of telomere length is essential in many aspects of cell biology, such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis. This product utilizes relative quantitative PCR to determine the average telomere length of the given sample. The kit uses a single copy gene located on human chromosome 11 as the reference for data normalization. The relative telomere length is determined by calculating the copy number ratio (T/S) of telomere repeat sequences (Tel) to the single-copy reference (SCR).
Storage Conditions	Nuclease-free ddH₂O store at 2-8°C, other components store at -20°C.
Notes	1. When storing or preparing the reaction system, avoid strong light exposure. Be sure to invert and mix before use. 2. The fluorescent signal in the reaction system can be standardized by adding ROX dye to the reaction system according to the selected instrument. Adjust volumes as appropriate to account for addition of the reference dye.
Shelf Life	1 year
Reactivity	Human

Product Details
Assay Type	Quantitative
Quality Control	The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by template serial dilution.
Samples	Genomic DNA
Components	2×qPCR master mix Telomere primer mix (10 μM) SCR primer mix (10 μM) 50×ROX dye Nuclease-free ddH₂O
Highlights	1. The primer set in the kit has passed the test to ensure: (1) efficient and reliable quantification and (2) no non-specific amplification. 2. Each set of primers has been verified by amplification curve efficiency (E>98%, R²>0.99), melting curve analysis, and gel electrophoresis verification. 3. The SYBR Green I dye in the master mix can analyze multiple target nucleic acids without the need to synthesize sequence-specific probes. 4. The hot start Taq DNA polymerase in the master mix can effectively inhibit the amplification caused by primer non-specific annealing. 5. Individually packaged ROX reference dyes for use in all real-time PCR instruments.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.