CAT. NO.: AC-02216
| General Info | |
|---|---|
| Size | 20 tests/50 tests |
| Product Overview | The real-time fluorescent qPCR (SYBR Green Real-time PCR) method is widely used in scientific research because of its excellent rapidity and quantification. Using this kit can accurately and easily analyze the expression of mRNA. The kit adopts the optimal reaction system based on SYBR Green qPCR Master Mix designed for fluorescent real-time quantitative PCR and Two-Step fluorescent realtime quantitative PCR, combined with specific primers for telomerase catalytic subunit gene TERT, which simplifies the experimental steps and improves experimental efficiency. Master Mix contains Maxima Hot Start Taq DNA Polymerase, dNTPS, optimized PCR reaction buffer, and SYBR Green dye. The use of Hot Start Taq DNA Polymerase provides PCR reactions with higher yield and sensitivity, specificity, and the ability to detect as few as 10 copies of the gene of interest. |
| Storage Conditions | -20°C |
| Notes | All methods for detecting telomerase cannot completely and quantitatively detect the activity of telomerase activity in cells, and the accuracy of the indirect relationship between the expression of the TERT gene or protein and telomerase needs further study. Customers who choose to use this kit already know the limitations by default. |
| Reactivity | Human |
| Product Details | |
|---|---|
| Assay Type | qPCR |
| Samples | DNA or RNA extracted from human cells |
| Components | Maxima SYBR Green qPCR Master Mix (2×) Water, nuclease-free Human TERT-F primer (1 OD) Human TERT-R primer (1 OD) GAPDH-F Primer (1 OD) GAPDH-R Primer (1 OD) |
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