CAT. NO.: AC-02216
| General Info | |
|---|---|
| Size | 20 tests/50 tests |
| Product Overview | The real-time fluorescent qPCR (SYBR Green Real-time PCR) method is extensively employed in scientific research due to its remarkable speed and precision in quantification. This kit offers a precise and straightforward means of analyzing mRNA expression. It leverages the meticulously designed optimal reaction system of SYBR Green qPCR Master Mix for fluorescent real-time quantitative PCR and Two-Step fluorescent real-time quantitative PCR, coupled with specific primers targeting the telomerase catalytic subunit gene TERT. This simplifies the experimental procedures and enhances overall efficiency. The Master Mix includes Maxima Hot Start Taq DNA Polymerase, dNTPs, an optimized PCR reaction buffer, and SYBR Green dye. The incorporation of Hot Start Taq DNA Polymerase results in PCR reactions with heightened yield, sensitivity, specificity, and the capability to detect as few as 10 copies of the target gene. |
| Storage Conditions | -20°C |
| Notes | Because all methods for detecting telomerase cannot completely and quantitatively detect the activity of telomerase in cells, and the accuracy of the indirect relationship between the expression of TERT gene or protein and telomerase needs further study, we hope that customers can choose to use it according to the actual situation. |
| Reactivity | Human |
| Product Details | |
|---|---|
| Assay Type | qPCR |
| Samples | Tissues, Cells, Blood Plasma and Serum |
| Components | Maxima SYBR Green qPCR Master Mix (2×) Water, nuclease-free Human TERT-F primer (1 OD) Human TERT-R primer (1 OD) GAPDH-F Primer (1 OD) GAPDH-R Primer (1 OD) |
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