CAT. NO.: AC-02217
| General Info | |
|---|---|
| Size | 24 tests/48 tests/96 tests |
| Product Overview | This assay utilizes the quantitative sandwich enzyme immunoassay technique. Initially, an antibody specific for TE is pre-coated onto a microplate. To perform the assay, standards, and samples are pipetted into the wells, where any TE present becomes bound by the immobilized antibody. After eliminating any unbound substances, a biotin-conjugated antibody that specifically recognizes TE is introduced into the wells. Following a subsequent washing step to remove unbound components, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. After another wash to eliminate any unbound avidin-enzyme reagent, a substrate solution is applied to the wells. In response, color development occurs, and the intensity of this color is directly proportional to the amount of TE initially bound in the first step of the assay. To complete the assay, the color development is halted, and the intensity of the color is measured, providing a quantifiable result reflecting the concentration of TE in the samples and standards. |
| Storage Conditions | 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the TERT ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Human |
| Product Details | |
|---|---|
| Assay Type | Quantitative Sandwich |
| Samples | Serum, Plasma, Tissue Homogenates |
| Detection Range | 12.5-800 ng/mL |
| Sensitivity | <3.12 ng/mL |
| Components | Assay plate (12×8 coated Microwells) Standard (Freeze dried) Biotin-antibody (100×concentrate) HRP-avidin (100×concentrate) Biotin-antibody Diluent HRP-avidin Diluent Sample Diluent Wash Buffer (25×concentrate) TMB Substrate Stop Solution Adhesive Strip (For 96 wells) Instruction manual |
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