CAT. NO.: TK105
| General Info | |
|---|---|
| Size | 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Product Overview | This product constitutes a ready-to-use microwell and strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the assessment of the Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG6) ELISA Kit target analytes within biological samples. The concentration gradients of the Kit standards or positive controls render a theoretical Kit detection range in biological research samples containing SMG6. The ELISA analytical biochemical technique of the Kit is based on SMG6 antibody-SMG6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SMG6 antigen targets in samples. It's important to note that this ELISA Kit is specifically tailored for detecting native, not recombinant SMG6. |
| Storage Conditions | Store all reagents at 2-8°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the EST1A ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Human |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Samples | Undiluted Body Fluids, Tissue Homogenates, Secretions |
| Target Info | |
|---|---|
| Molecular Weight | 160,462 Da |
| NCBI Accession | NP_001243756.1 |
| NCBI GenBank Nucleotide | NM_001256827.1 |
| NCBI GeneID | 23293 |
| NCBI Official Full Name | telomerase-binding protein EST1A isoform 3 |
| NCBI Protein Information | telomerase-binding protein EST1A, telomerase subunit EST1A, ever shorter telomeres 1A, EST1 telomerase component homolog A, smg-6 homolog, nonsense mediated mRNA decay factor |
| OMIM | 610963 |
| UniProt Entry Name | EST1A_HUMAN |
| UniProt Gene Name | EST1A |
| UniProt Protein Name | Telomerase-binding protein EST1A |
| UniProt Synonym Gene Names | C17orf31, EST1A, KIAA0732 |
| UniProt Synonym Protein Names | EST1-like protein A, Ever shorter telomeres 1A, Smg-6 homolog, Telomerase subunit EST1A, hSmg5/7a |
| UniProt Primary Accession | Q86US8 |
| UniProt Secondary Accession | O94837, Q86VH6, Q9UF60, B7Z874 |
| Protein Family | Telomerase-binding protein |
| NCBI Summary | This gene encodes a component of the telomerase ribonucleoprotein complex responsible for the replication and maintenance of chromosome ends. The encoded protein also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD. Alternatively spliced transcript variants encoding distinct protein isoforms have been described. [provided by RefSeq, Feb 2014] |
| UniProt Comments | EST1A: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'- (GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER). 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-binding; Nucleolus; Hydrolase; EC 3.1.-.-Chromosomal Location of Human Ortholog: 17p13.3Cellular Component: chromosome, telomeric region; cytoplasm; telomerase holoenzyme complex; nucleolus; nucleus; cytosolMolecular endoribonuclease activity; protein binding; metal ion binding; telomeric DNA bindingBiological Process: mRNA export from nucleus; regulation of dephosphorylation; mRNA catabolic process, nonsense-mediated decay; gene expression; telomere maintenance |
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