Human Protection of Telomeres Protein 1 (POT1) ELISA Kit

INQUIRY

CAT. NO.: TK102

General Info
Size	24 Tests / 48 Tests / 96 Tests / 5×96 Tests / 10×96 Tests
Application	Enzyme-linked immunosorbent assay for antigen detection.
Product Overview	This ELISA Kit employs the Sandwich-ELISA method. The micro ELISA plate provided in this Kit has been pre-coated with an antibody specific to POT1. To conduct the assay, standards or samples are introduced into the respective micro ELISA plate wells and mixed with the specific antibody. Then a biotinylated detection antibody specific for POT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each microplate well successively and incubated. Unbound components are then thoroughly washed away. Following this, a substrate solution is introduced to each well. Only those wells that contain POT1, biotinylated detection antibody, and Avidin-HRP conjugate will appear blue in color. The enzymatic reaction with the substrate is halted by adding a sulfuric acid solution, causing the color to change to yellow. The optical density (OD) is quantitatively measured using a spectrophotometer at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of POT1. You can determine the POT1 in the samples by comparing the OD of the samples to the standard curve.
Storage Conditions	Store at 4°C
Notes	Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the POT1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation.
Reactivity	Human
Specificity	This kit recognizes natural and recombinant Human POT1. No significant cross-reactivity or interference between Human POT1 and analogues was observed.
Shipping Conditions	Dry Ice

Product Details
Assay Type	Sandwich
Samples	Serum, Plasma, Biological Fluids
Detection Range	0.313-20 ng/mL
Sensitivity	Min: 0.188 ng/mL; Max: 20 ng/mL
Inter-assay CV	CV<12%
Intra-assay CV	CV<10%
Detection Wavelength	450 nm

Target Info
Molecular Weight	60,534 Da
NCBI Accession	NP_056265.2
NCBI GenBank Nucleotide	NM_015450.2
NCBI GeneID	25913
NCBI Official Full Name	protection of telomeres protein 1 isoform 1
NCBI Official Symbol	POT1
NCBI Official Synonym Full Names	protection of telomeres 1
NCBI Official Synonym Symbols	CMM10, HPOT1
NCBI Protein Information	protection of telomeres protein 1, POT1-like telomere end-binding protein, protection of telomeres 1 homolog
OMIM	606478
UniProt Entry Name	POTE1_HUMAN
UniProt Gene Name	POT1
UniProt Protein Name	Protection of telomeres protein 1
UniProt Synonym Gene Names	hPot1
UniProt Synonym Protein Names	POT1-like telomere end-binding protein
UniProt Primary Accession	Q9NUX5
UniProt Secondary Accession	O95018, Q5MJ36, Q9H662, Q9NW19, Q9UG95
Protein Family	Potassium transporter
NCBI Summary	This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
UniProt Comments	POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 7q31.33Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleusMolecular protein binding; single-stranded telomeric DNA binding; DEAD/H-box RNA helicase bindingBiological Process: positive regulation of helicase activity; negative regulation of telomerase activity; telomere assembly; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere maintenance; DNA duplex unwinding; positive regulation of telomere maintenance via telomeraseDisease: Melanoma, Cutaneous Malignant, Susceptibility To, 10

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