CAT. NO.: TK102
| General Info | |
|---|---|
| Size | 24 Tests / 48 Tests / 96 Tests / 5×96 Tests / 10×96 Tests |
| Application | Enzyme-linked immunosorbent assay for antigen detection. |
| Product Overview | This ELISA Kit employs the Sandwich-ELISA method. The micro ELISA plate provided in this Kit has been pre-coated with an antibody specific to POT1. To conduct the assay, standards or samples are introduced into the respective micro ELISA plate wells and mixed with the specific antibody. Then a biotinylated detection antibody specific for POT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each microplate well successively and incubated. Unbound components are then thoroughly washed away. Following this, a substrate solution is introduced to each well. Only those wells that contain POT1, biotinylated detection antibody, and Avidin-HRP conjugate will appear blue in color. The enzymatic reaction with the substrate is halted by adding a sulfuric acid solution, causing the color to change to yellow. The optical density (OD) is quantitatively measured using a spectrophotometer at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of POT1. You can determine the POT1 in the samples by comparing the OD of the samples to the standard curve. |
| Storage Conditions | Store at 4°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the POT1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Human |
| Specificity | This kit recognizes natural and recombinant Human POT1. No significant cross-reactivity or interference between Human POT1 and analogues was observed. |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Sandwich |
| Samples | Serum, Plasma, Biological Fluids |
| Detection Range | 0.313-20 ng/mL |
| Sensitivity | Min: 0.188 ng/mL; Max: 20 ng/mL |
| Inter-assay CV | CV<12% |
| Intra-assay CV | CV<10% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 60,534 Da |
| NCBI Accession | NP_056265.2 |
| NCBI GenBank Nucleotide | NM_015450.2 |
| NCBI GeneID | 25913 |
| NCBI Official Full Name | protection of telomeres protein 1 isoform 1 |
| NCBI Official Symbol | POT1 |
| NCBI Official Synonym Full Names | protection of telomeres 1 |
| NCBI Official Synonym Symbols | CMM10, HPOT1 |
| NCBI Protein Information | protection of telomeres protein 1, POT1-like telomere end-binding protein, protection of telomeres 1 homolog |
| OMIM | 606478 |
| UniProt Entry Name | POTE1_HUMAN |
| UniProt Gene Name | POT1 |
| UniProt Protein Name | Protection of telomeres protein 1 |
| UniProt Synonym Gene Names | hPot1 |
| UniProt Synonym Protein Names | POT1-like telomere end-binding protein |
| UniProt Primary Accession | Q9NUX5 |
| UniProt Secondary Accession | O95018, Q5MJ36, Q9H662, Q9NW19, Q9UG95 |
| Protein Family | Potassium transporter |
| NCBI Summary | This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| UniProt Comments | POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 7q31.33Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleusMolecular protein binding; single-stranded telomeric DNA binding; DEAD/H-box RNA helicase bindingBiological Process: positive regulation of helicase activity; negative regulation of telomerase activity; telomere assembly; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere maintenance; DNA duplex unwinding; positive regulation of telomere maintenance via telomeraseDisease: Melanoma, Cutaneous Malignant, Susceptibility To, 10 |
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