CAT. NO.: TK103
| General Info | |
|---|---|
| Size | 24-Strip-Wells / 48-Strip-Wells / 96-Strip-Wells / 5×96-Strip-Wells / 10×96-Strip-Wells |
| Application | Enzyme-linked immunosorbent assay for antigen detection. |
| Product Overview | The test principle applied in this Kit is Sandwich enzyme immunoassay. The microtiter plate provided in this Kit has been pre-coated with an antibody specific to Protection Of Telomeres 1 Homolog (POT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protection Of Telomeres 1 Homolog (POT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protection Of Telomeres 1 Homolog (POT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protection Of Telomeres 1 Homolog (POT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Storage Conditions | Store at 4°C |
| Notes | Occasionally, trace amounts of reagent may remain in the closure of the product vial during shipment and storage of the POT1 ELISA kit. It is recommended that any liquid that may be present in the lid of the container be centrifuged to the bottom using a bench-top centrifuge prior to experimentation. |
| Reactivity | Human |
| Specificity | This assay has high sensitivity and excellent specificity for detection of protection of Telomeres 1 Homolog (POT1). No significant cross-reactivity or interference between Protection of Telomeres 1 Homolog (POT1) and analogues was observed. |
| Shipping Conditions | Dry Ice |
| Product Details | |
|---|---|
| Assay Type | Double-antibody Sandwich |
| Samples | Tissue Homogenates, Cell Lysates, Other Biological Fluids |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | < 0.118 ng/mL |
| Inter-assay CV | CV<12% |
| Intra-assay CV | CV<10% |
| Detection Wavelength | 450 nm |
| Target Info | |
|---|---|
| Molecular Weight | 60,534 Da |
| NCBI Accession | NP_056265.2 |
| NCBI GenBank Nucleotide | NM_015450.2 |
| NCBI GeneID | 25913 |
| NCBI Official Full Name | protection of telomeres protein 1 isoform 1 |
| NCBI Official Symbol | POT1 |
| NCBI Official Synonym Full Names | protection of telomeres 1 |
| NCBI Official Synonym Symbols | GLM9, CMM10, HPOT1 |
| NCBI Protein Information | protection of telomeres protein 1 |
| OMIM | AY823520 mRNA |
| UniProt Gene Name | POT1 |
| UniProt Protein Name | Protection of telomeres protein 1 |
| UniProt Synonym Gene Names | hPot1 |
| UniProt Synonym Protein Names | POT1-like telomere end-binding protein |
| UniProt Primary Accession | Q9NUX5 |
| UniProt Secondary Accession | O95018, Q5MJ36, Q9H662, Q9NW19, Q9UG95 |
| Protein Family | Potassium transporter |
| NCBI Summary | This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| UniProt Comments | Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. |
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