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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Human Cellular Senescence Real-time PCR Arrays

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CAT. NO.: PA001

General Info
Size	96-well plate/384-well plate
Product Overview	We offer real-time PCR arrays for highly specific, reliable, and reproducible results for signaling pathways analysis, even with small amounts of precious samples like FFPE or LCM samples. Real-time PCR arrays in 96-well or 384-well plate formats are compatible with most of all qPCR instruments on the market. Each 96-well plate allows to analyze 84 genes of interest, specific to a signaling or pathological pathway with 8 reference genes necessary to the robust normalization step of your qPCR data and 4 quality controls. All signaling pathways are validated at the experimental level on a large collection of tissues and cell lines on a high-throughput molecular platform, thanks to stringent and strong quality control criteria to guarantee you the best results.
Storage Conditions	Store at -20°C
Notes	Do not use DEPC H2O. cDNA diluted at the 1/12 from a Reverse Transcription (20 µL) performed with 1 μg of RNA (for one qPCR 96 plate) or equivalent depending on the used Reverse Transcription kit. Diluted cDNA at 1/6 with ultra-pure H2O from a Reverse Transcription (20 µL) performed with 0.5 μg of RNA (for 1/4 qPCR 384 plate, as 4 cDNA qPCR mixes are required to fill an entire qPCR 384 plate) or equivalent depending on the used Reverse Transcription kit.
Reactivity	Human
Shipping Conditions	Transport at 4°C

Product Details
Quality Control	The qPCR efficiency of your experiment : Cq values for QC1 & QC2 wells should be between 26 and 30, according to qPCR instruments and the choice of automatic or manual Cq analysis method. Intra-plate repeatability of the qPCR results obtained in the 2 wells (duplicates) : the difference between the 2 Cq values (ΔCq) for QC1 and QC2 wells have to be less than 0.5. Inter-plate repeatability of the qPCR results obtained with several plates: the difference between the mean of Cq values for QC1 and QC2 wells of each plate have to be less than 1. A negative control in duplicates (QC3 & QC4 wells) : Cq values for QC3 & QC4 wells have to be higher than 35 to guarantee the absence of reaction mix contamination.
Highlights	1. All-in-one solution. 2. Rigorous intron flanking primer design. 3. Fast and easy protocol.

Product Details

Quality Control

The qPCR efficiency of your experiment : Cq values for QC1 & QC2 wells should be between 26 and 30, according to qPCR instruments and the choice of automatic or manual Cq analysis method.
Intra-plate repeatability of the qPCR results obtained in the 2 wells (duplicates) : the difference between the 2 Cq values (ΔCq) for QC1 and QC2 wells have to be less than 0.5.
Inter-plate repeatability of the qPCR results obtained with several plates: the difference between the mean of Cq values for QC1 and QC2 wells of each plate have to be less than 1.
A negative control in duplicates (QC3 & QC4 wells) : Cq values for QC3 & QC4 wells have to be higher than 35 to guarantee the absence of reaction mix contamination.

Highlights

1. All-in-one solution.
2. Rigorous intron flanking primer design.
3. Fast and easy protocol.

Target Info
Gene Primer List	AKT1, MORC3, PTEN, ALDH1A3, CALR, IGFBP3, MYC, NFKB1, PIK3CA, SERPINB2, CD44, COL1A1, COL3A1, FN1, TGFB1, TGFB1I1, THBS1, CCNB1, CCND1, CCNE1, CDC25C, CDK2, CDK4, CDK6, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, CITED2, HRAS, IFNG, ING1, GLB1, GSK3B, SERPINE1, ATM, GADD45A, NBN, PCNA, RBL2, TERF2, TERT, TP53BP1, CCNA2, CDKN1B, TP53, IGF1, IGF1R, IGFBP5, IGFBP7, MAP2K1, MAP2K3, MAP2K6, MAPK14, E2F1, E2F3, ETS1, ETS2, ID1, MDM2, RB1, NOX4, PRKCD, SOD1, SOD2, BMI1, IFNB1, ABL1, RBL1, SIRT1, TBX2, TBX3, TWIST1, CDKN1A, CHEK1, CHEK2, PLAU, SPARC, VIM, EGR1, IRF3, IRF5, IRF7.

Target Info

Gene Primer List

AKT1, MORC3, PTEN, ALDH1A3, CALR, IGFBP3, MYC, NFKB1, PIK3CA, SERPINB2, CD44, COL1A1, COL3A1, FN1, TGFB1, TGFB1I1, THBS1, CCNB1, CCND1, CCNE1, CDC25C, CDK2, CDK4, CDK6, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, CITED2, HRAS, IFNG, ING1, GLB1, GSK3B, SERPINE1, ATM, GADD45A, NBN, PCNA, RBL2, TERF2, TERT, TP53BP1, CCNA2, CDKN1B, TP53, IGF1, IGF1R, IGFBP5, IGFBP7, MAP2K1, MAP2K3, MAP2K6, MAPK14, E2F1, E2F3, ETS1, ETS2, ID1, MDM2, RB1, NOX4, PRKCD, SOD1, SOD2, BMI1, IFNB1, ABL1, RBL1, SIRT1, TBX2, TBX3, TWIST1, CDKN1A, CHEK1, CHEK2, PLAU, SPARC, VIM, EGR1, IRF3, IRF5, IRF7.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.