Human Age-associated ELOVL2 Promoter Methylation Quantification qPCR Assay Kit

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CAT. NO.: SMT008

General Info
Size	100 tests
Product Overview	DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors including aging. The CpG island methylation level at human ELOVL2 gene promoter has been well-associated with chronological age in various populations, cell types, and tissues. ELOVL2 promoter methylation level is therefore a potential marker for human chronological age. ScienCell's Human Age-associated ELOVL2 Promoter Methylation Quantification qPCR Assay Kit (HAEPM) is designed to quantify the level of human ELOVL2 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

General Info

Size

100 tests

Product Overview

DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors including aging. The CpG island methylation level at human ELOVL2 gene promoter has been well-associated with chronological age in various populations, cell types, and tissues. ELOVL2 promoter methylation level is therefore a potential marker for human chronological age. ScienCell's Human Age-associated ELOVL2 Promoter Methylation Quantification qPCR Assay Kit (HAEPM) is designed to quantify the level of human ELOVL2 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

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