CAT. NO.: DMK021
| General Info | |
|---|---|
| Size | 48 tests/96 tests |
| Product Overview | Histone H3 (di-methyl K4) Quantification Kit (Colorimetric, Circulating) is a convenient package of tools designed to specifically measure circulating dimethyl histone H3K4 (H3K4me2) from biological fluid samples such as plasma and serum from human, mouse or rat. This kit only recognizes H3K4me2 with no cross-reactivity with unmodified H3 or other modifications at the same lysine site. The amount of plasma or serum for each assay can be 10-40 µL with an optimal amount of 30 µL. |
| Notes | Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic gene inactivation is methylation of CpG islands in genomic DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcriptional repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. NSD3 is the major histone methyltransferase that catalyzes dimethylation of histone H3 at lysine 4 (H3-K4) in mammalian cells. LSD2 and JARIDs are the major histone demethylase that demethylates H3K4. H3K4me2 has been viewed as a signature mark of highly transcribed genes, which is placed exclusively in the 5’- region downstream of the promoter. The H3K4me2 can also be changed by inhibition or activation of HMTs. Circulating histone H3K4me2 in plasma or serum has been observed and demonstrated as the marker for many different diseases or pathological changes such as cancer progression. Therefore, detection of circulating H3K4me2 would provide useful information for a better understanding of epigenetic regulation of gene activation and silencing, histone modification-associated pathological processes, screening of disease-related biomarkers, as well as for developing histone modification-targeted drugs. |
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