Granzyme B (human) ELISA Kit

INQUIRY

CAT. NO.: SAT-048

General Info
Size	100 tests
Product Overview	Granzyme B is a serine protease expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and functions in cell-mediated immunity. Granzyme B (human) ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human Granzyme B is coated on a 96-well plate. Standards and test samples are added to the wells and Granzyme B present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Granzyme B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Granzyme B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/mL – 1000 pg/mL. Sensitivity: < 10 pg/mL.
Notes	This ELISA kit is used for quantitative protein detection, establishing normal range, validation of antibody array results.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.