GPNMB (E7U1Z) Rabbit Antibody

INQUIRY

CAT. NO.: AMA-0786

Target Information
UniProt ID	Q99P91

Product Details
Size	100 µL
Reactivity	Mouse
Host	Rabbit
Source/Isotype	Rabbit IgG
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse GPNMB protein.

Usage
Application	WB, IF, F
Product Usage Information Dilution	Western Blotting (1:1000); Immunofluorescence (1:400); Flow Cytometry (1:50-1:200)
MW (Target)	90 kDa

Background
Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein overexpressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes regulating osteoblast and osteoclast differentiation and function stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells enhancing autophagosome fusion to lysosomes in tissue repair, and regulating degradation of cellular debrisWhile typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers. GPNMB is typically localized to intracellular compartments in normal cells but investigators found it primarily on the cell surface of tumor cells. Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target.The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface. Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration.

Background

Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein overexpressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes regulating osteoblast and osteoclast differentiation and function stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells enhancing autophagosome fusion to lysosomes in tissue repair, and regulating degradation of cellular debrisWhile typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers. GPNMB is typically localized to intracellular compartments in normal cells but investigators found it primarily on the cell surface of tumor cells. Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target.The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface. Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration.

Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our products, please contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.