GAK (E7W7Z) Rabbit Antibody

INQUIRY

CAT. NO.: AMA-0666

Target Information
UniProt ID	O14976

Product Details
Size	100 µL
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Source/Isotype	Rabbit IgG
Storage	Store at -20°C. Do not aliquot the antibody.
Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro758 of human GAK protein.

Usage
Application	WB, IP
Product Usage Information Dilution	Western Blotting (1:1000); Immunoprecipitation (1:50)
MW (Target)	175 kDa

Background
Cyclin-G-associated kinase (GAK) is the ubiquitous form of the neuronal-specific protein auxilin 1 and is an essential cofactor for Hsc70-dependent uncoating of clathrin-coated vesicles in non-neuronal cells The recruitment of GAK to clathrin-coated pits during endocytosis is dependent on its PTEN-like domain, which has been shown to bind to phospholipids It has been postulated that the JAK inhibitor baricitinib may impair entry of the SARS-CoV-2 virus by inhibiting AAK1- and GAK-mediated endocytosis via ACE2 In the nucleus, GAK forms complexes with cyclin G1 and other nuclear proteins and plays an essential role in proper mitotic progression by maintaining centrosome structure GAK is also required for the regulation of basal, PRKN-independent mitophagy by modifying the mitochondrial network and lysosomal morphology that compromise efficient transport of mitochondria for degradation.

Background

Cyclin-G-associated kinase (GAK) is the ubiquitous form of the neuronal-specific protein auxilin 1 and is an essential cofactor for Hsc70-dependent uncoating of clathrin-coated vesicles in non-neuronal cells The recruitment of GAK to clathrin-coated pits during endocytosis is dependent on its PTEN-like domain, which has been shown to bind to phospholipids It has been postulated that the JAK inhibitor baricitinib may impair entry of the SARS-CoV-2 virus by inhibiting AAK1- and GAK-mediated endocytosis via ACE2 In the nucleus, GAK forms complexes with cyclin G1 and other nuclear proteins and plays an essential role in proper mitotic progression by maintaining centrosome structure GAK is also required for the regulation of basal, PRKN-independent mitophagy by modifying the mitochondrial network and lysosomal morphology that compromise efficient transport of mitochondria for degradation.

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