Fast Bisulfite Conversion Kit

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CAT. NO.: DMK028

General Info
Size	50 tests
Product Overview	Fast DNA modification Kit allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil. DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases. Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same. Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

General Info

Size

50 tests

Product Overview

Fast DNA modification Kit allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.

Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

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All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.