CAT. NO.: AMA-0207
| Product Details | |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Source/Isotype | Rabbit IgG |
| Storage | Store at -20°C. |
| Purification | Affinity purified |
| Usage | |
|---|---|
| Application | WB |
| Product Usage Information Dilution | Western Blotting (0.5-4 µg/mL); Immunohistochemistry (10-20 µg/mL) |
| Background | |
|---|---|
| eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroμghly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation. | |
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